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首页> 外文期刊>Progress in Biomaterials >Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes
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Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes

机译：蚕丝丝胶作为生物材料的评估：人角膜缘上皮细胞在家蚕丝胶蛋白膜上的体外生长

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Sericin and fibroin are the two major proteins in the silk fibre produced by the domesticated silkworm, Bombyx mori. Fibroin has been extensively investigated as a biomaterial. We have previously shown that fibroin can function successfully as a substratum for growing cells of the eye. Sericin has been so far neglected as a biomaterial because of suspected allergenic activity. However, this misconception has now been dispelled, and sericin’s biocompatibility is currently indisputable. Aiming at promoting sericin as a possible substratum for the growth of corneal cells in order to make tissue-engineered constructs for the restoration of the ocular surface, in this study we investigated the attachment and growth in vitro of human corneal limbal epithelial cells (HLECs) on sericin-based membranes. Sericin was isolated and regenerated from the silkworm cocoons by an aqueous procedure, manufactured into membranes, and characterized (mechanical properties, structural analysis, contact angles). Primary cell cultures from two donors were established in serum-supplemented media in the presence of murine feeder cells. Membranes made of sericin and fibroin-sericin blends were assessed in vitro as substrata for HLECs in a serum-free medium, in a cell attachment assay and in a 3-day cell growth experiment. While the mechanical characteristics of sericin were found to be inferior to those of fibroin, its ability to enhance the attachment of HLECs was significantly superior to fibroin, as revealed by the PicoGreen? assay. Evidence was also obtained that cells can grow and differentiate on these substrata.
机译：丝胶和丝素蛋白是家蚕家蚕产生的蚕丝纤维中的两种主要蛋白质。纤维蛋白已被广泛地研究为生物材料。先前我们已经证明纤维蛋白可以成功地用作眼细胞生长的基质。迄今为止，由于怀疑有变应原活性，丝胶已被忽略为生物材料。但是，这种错误观念现在已经消除，丝胶蛋白的生物相容性目前无可争议。为了促进丝胶蛋白作为角膜细胞生长的可能基质，以便制造组织工程的结构以恢复眼表，在这项研究中，我们研究了人角膜缘角膜缘上皮细胞（HLEC）的体外附着和生长在丝胶蛋白膜上通过水溶过程从蚕茧中分离并回收丝胶蛋白，将其制成膜并进行表征（机械性能，结构分析，接触角）。在鼠饲养细胞的存在下，在补充血清的培养基中建立来自两个供体的原代细胞培养。在无血清培养基中，细胞附着试验和三天的细胞生长实验中，将丝胶蛋白和丝蛋白-丝胶蛋白混合物制成的膜在体外评估为HLEC的基质。虽然发现丝胶蛋白的机械特性不如丝蛋白，但其增强HLEC附着的能力明显优于丝蛋白，如PicoGreen？所揭示的那样。分析。还获得了细胞可以在这些基质上生长和分化的证据。

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