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首页> 外文期刊>Proteomes >Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment
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Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment

机译：伴侣亲和富集从乳腺癌细胞株的细胞外囊泡的差异蛋白质组学分析。

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The complexity of human tissue fluid precludes timely identification of cancer biomarkers by immunoassay or mass spectrometry. An increasingly attractive strategy is to primarily enrich extracellular vesicles (EVs) released from cancer cells in an accelerated manner compared to normal cells. The Vn96 peptide was herein employed to recover a subset of EVs released into the media from cellular models of breast cancer. Vn96 has affinity for heat shock proteins (HSPs) decorating the surface of EVs. Reflecting their cells of origin, cancer EVs displayed discrete differences from those of normal phenotype. GELFrEE LC/MS identified an extensive proteome from all three sources of EVs, the vast majority having been previously reported in the ExoCarta database. Pathway analysis of the Vn96-affinity proteome unequivocally distinguished EVs from tumorigenic cell lines (SKBR3 and MCF-7) relative to a non-tumorigenic source (MCF-10a), particularly with regard to altered metabolic enzymes, signaling, and chaperone proteins. The protein data sets provide valuable information from material shed by cultured cells. It is probable that a vast amount of biomarker identities may be collected from established and primary cell cultures using the approaches described here.
机译：人类组织液的复杂性使得无法通过免疫测定或质谱法及时鉴定癌症生物标志物。与正常细胞相比，一种越来越有吸引力的策略是主要以加速方式富集从癌细胞释放的细胞外囊泡（EVs）。 Vn96肽在本文中用于回收从乳腺癌细胞模型释放到培养基中的EV的子集。 Vn96对装饰电动汽车表面的热激蛋白（HSP）具有亲和力。反映其起源的细胞，癌症电动汽车与正常表型显示出离散的差异。 GELFrEE LC / MS从所有三种电动汽车来源中鉴定出了广泛的蛋白质组，其中绝大部分以前已在ExoCarta数据库中报告过。相对于非致瘤性来源（MCF-10a），Vn96亲和性蛋白质组的EV明确区别于致瘤细胞系（SKBR3和MCF-7）的途径，特别是关于代谢酶，信号传导和伴侣蛋白的改变。蛋白质数据集提供了来自培养细胞脱落的物质的有价值的信息。使用此处描述的方法，很可能可以从既定的和原代细胞培养物中收集大量的生物标记物身份。

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