Peptide sequencing using mass spectrometry: a practical guide

Cantú Marcelo Delmar; Carrilho Emanuel; Fundo de Defesa da Citricultura Araraquara Brasil; Palma Mario Sérgio; Universidade de S?o Paulo S?o Carlos Brasil; Universidade Estadual Paulista Rio Claro Brasil; Wulff Nelson Arno

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Peptide sequencing using mass spectrometry: a practical guide

机译：使用质谱进行肽测序：实用指南

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This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.

机译：本文介绍了应用于蛋白质组学的肽段质谱解释的基础知识，并面向化学家，生物化学家和生物学家。该手稿提供了详细的协议，旨在作为了解肽序的首选指南。本教程是根据书目的详尽修订和作者的经验精心编写的。为了证明所提出的指南的适用性，通过应用提出的合理性已经成功地解释了在不同仪器上获得的光谱。

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《Quimica nova》 |2008年第3期|共页
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Cantú Marcelo Delmar; Carrilho Emanuel; Fundo de Defesa da Citricultura Araraquara Brasil; Palma Mario Sérgio; Universidade de S?o Paulo S?o Carlos Brasil; Universidade Estadual Paulista Rio Claro Brasil; Wulff Nelson Arno;
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1. Paired single residue-transposed Lys-N and Lys-C digestions for label-free identification of N-terminal and C-terminal MS/MS peptide product ions: ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry for peptide de novo sequencing [J] . Brownstein Naomi C., Guan Xiaoyan, Mao Yuan, Rapid Communications in Mass Spectrometry: RCM . 2015,第7期

机译：成对的单残基转座的Lys-N和Lys-C消化可无标记地鉴定N末端和C末端MS / MS肽产物离子：超高分辨率傅里叶变换离子回旋共振质谱和串联质谱从头开始排序
2. Bromine isotopic signature facilitates de novo sequencing of peptides in free-radical-initiated peptide sequencing (FRIPS) mass spectrometry [J] . Nam Jungjoo, Kwon Hyuksu, Jang Inae, Journal of mass spectrometry: JMS . 2015,第2期

机译：溴同位素标记有助于在自由基引发的肽测序（FRIPS）质谱中对肽进行从头测序
3. De novo sequencing, peptide composition analysis, and composition-based sequencing: A new strategy employing accurate mass determination by Fourier transform ion cyclotron resonance mass spectrometry [J] . Spengler B Journal of the American Society for Mass Spectrometry . 2004,第5期

机译：从头测序，肽成分分析和基于成分的测序：采用傅立叶变换离子回旋共振质谱进行精确质量测定的新策略
4. De novo sequencing of peptides in free radical initiated peptide sequencing(FRIPS) mass spectrometry [C] . Han Bin Oh ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：肽在自由基引发肽测序（FRIPS）质谱中的肽测序
5. Development and Application of Methodologies to Facilitate Peptide Sequencing with Mass Spectrometry [D] . English, Ann Michelle 2011

机译：质谱促进肽测序的方法学的发展与应用
6. Peptide sequencing by fast atom bombardment mass spectrometry: acid hydrolysis or tandem mass spectrometry? [O] . F de Angelis, M Botta, R Nicoletti 1987

机译：通过快速原子轰击质谱进行肽测序：酸水解还是串联质谱？
7. Peptide sequencing using mass spectrometry: a practical guide [O] . Cantú, Marcelo Delmar, Carrilho, Emanuel, Wulff, Nelson Arno, 2008

机译：使用质谱进行肽测序：实用指南
8. High Resolution Electrospray Mass Spectrometry with a Magnetic Sector Instrument:Accurate Mass Measurement and Peptide Sequencing [R] . D'Agostino, P. A., Hancock, J. R., Provost, L. R., 1995

机译：采用磁性扇形仪器的高分辨率电喷雾质谱：精确质量测量和肽测序

Peptide sequencing using mass spectrometry: a practical guide

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