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Stir-bar sorptive extraction for drugs analysis in biological fluids

机译:搅拌棒吸附萃取用于生物液体中的药物分析

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A novel solventless sample preparation, stir-bar sorptive extraction (SBSE), for extraction, and sample enrichment of organic compounds from biological fluids, is described in this manuscript from principle to applications. The SBSE is based on sorptive extraction, whereby the compounds are extracted into a polymer coating, polydimethylsiloxane (PDMS), on a magnetic stirring rod. The extraction is controlled by the partitioning coefficient of drugs between the PDMS and sample matrix, and upon the sample-extraction medium phase ratio. The SBSE technique has been applied successfully, with high sensitivities, to biomedical analysis of volatiles and for semi-volatiles drugs from biological sample, including urine, plasma, and saliva. SBSE combined with in situ derivatization, drugs quite more polar (e.g. metabolites) also can be analyzed.
机译:该手稿从原理到应用都介绍了一种新颖的无溶剂样品制备,搅拌棒吸附萃取(SBSE),用于从生物流体中萃取和富集有机化合物。 SBSE是基于吸附萃取的,由此将化合物萃取到磁力搅拌棒上的聚合物涂层聚二甲基硅氧烷(PDMS)中。提取取决于药物在PDMS与样品基质之间的分配系数,并取决于样品提取介质的相比。 SBSE技术已经以高灵敏度成功地应用于挥发性物质的生物医学分析以及生物样品(包括尿液,血浆和唾液)中的半挥发性药物的分析。 SBSE与原位衍生化相结合,还可以分析极性更大的药物(例如代谢产物)。

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