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首页> 外文期刊>Química Nova >Aplica??o da CLAE para determina??o do ácido 10-Hidróxi-2-decenóico (10-HDA) em geléia real pura e adicionada a mel brasileiro
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Aplica??o da CLAE para determina??o do ácido 10-Hidróxi-2-decenóico (10-HDA) em geléia real pura e adicionada a mel brasileiro

机译:应用CLAE测定纯蜂王浆中的10-羟基-2-癸烯酸(10-HDA)并添加到巴西蜂蜜中

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The aim of this paper was to determine the 10-HDA in pure royal jelly and products containing royal jelly, using HPLC methodology. 10-HDA is the natural indicator of the presence of royal jelly in products and also gives the authenticity of pure royal jelly. The chromatographic conditions used were: isocratic system, C18-H column, auto sampler, diode array UV-VIS detector (225 nm), mobile phase with methanol/water (45:55), pH= 2.5 and a-naphtol as internal standard. The results obtained using laboratory samples for pure royal jelly were 2.37%, varying from 0.15% for honey with 10% of royal jelly to 2.10% for honey with 90% of royal jelly respectivelly. For commercial products, the 10-HDA content varied from no detectable to 0.026%. The recovery test presented a minumum of 100.44% The detection limit was 45.92 ng/mL and the quantification limit was 76.53 ng/mL.
机译:本文的目的是使用HPLC方法测定纯蜂王浆和含有蜂王浆的产品中的10-HDA。 10-HDA是产品中蜂王浆存在的自然指标,并且还提供了纯蜂王浆的真实性。使用的色谱条件为:等度系统,C18-H柱,自动进样器,二极管阵列UV-VIS检测器(225 nm),流动相,甲醇/水(45:55),pH = 2.5和α-萘酚作为内标。使用纯净蜂王浆的实验室样品获得的结果为2.37%,范围从含10%蜂王浆的蜂蜜的0.15%到含90%蜂王浆的蜂蜜的2.10%不等。对于商业产品,10-HDA含量从无法检测到0.026%。回收率测试的最低要求为100.44%。检测限为45.92 ng / mL,定量限为76.53 ng / mL。

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    《Química Nova》 |2003年第5期|共4页
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