Transmembrane Ca(2+) exchange in depolarized rat myometrium mitochondria

L. G. Babich; S. G. Shlykov; N. V. Kandaurova; S. A. Kosterin

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Transmembrane Ca(2+) exchange in depolarized rat myometrium mitochondria

机译：跨膜Ca（2+）交换去极化大鼠子宫肌层线粒体中。

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Polarization of the inner membrane is the key factor in maintenance of the physiologically significant cations accumulation, in particular Casup2+/sup, in the mitochondria. It has been well established that mitochondria accumulate calcium through the uniporter, driven by the mitochondrial membrane potential. Nevertheless, it has been shown that depolarized mitochondria also accumulate Casup2+/sup. The aim of this paper is to investigate free Ca level in depolarized myometrium mitochondria. As we have shown previously Casup2+/sup addition to the incubation medium, that did not contain K-phosphate, ATP and Mgsup2+/sup, led to inner mitochondrial membrane depolarization. Nevertheless Casup2+/sup addition to such medium led to the concentration-dependent accumulation of this cation in the matrix. RuR or Mg addition to the incubation medium led to the higher elevation of mitochondrial Casup2+/sup level in depolarized mitochondria. Mitochondrial Casup2+/sup level was not affected by 5 μM cyclosporine A. It was suggested that Нsup+/sup/Саsup2+/sup exchanger could provide calcium accumulation in depolarized mitochondria. The elevation of mitochondrial Casup2+/sup level after addition of Mgsup2+/sup and RuR may be due to inhibition of Casup2+/sup efflux through Casup2+/sup uniporter.

机译：内膜的极化是维持线粒体中生理上重要的阳离子，尤其是Ca 2 + 的关键因素。公认的是，线粒体在线粒体膜电位的驱动下通过单向转运体积累钙。然而，已经证明去极化的线粒体也积累Ca 2 + 。本文的目的是研究去极化的子宫肌层线粒体中的游离钙水平。如我们先前所显示的，向不含K-磷酸盐，ATP和Mg 2 + 的孵育培养基中添加Ca 2 + 导致内部线粒体膜去极化。尽管如此，向此类培养基中添加Ca 2 + 仍导致该阳离子在基质中的浓度依赖性累积。向培养液中添加RuR或Mg导致去极化线粒体中线粒体Ca 2 + 的升高。线粒体Ca 2 + 的水平不受5μM环孢霉素A的影响。提示Н + /Са 2 + 交换子可以提供钙积累在去极化的线粒体中。添加Mg 2 + 和RuR后线粒体Ca 2 + 的升高可能是由于Ca 2 + 外排通过Ca < sup> 2 + 单端口。

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《Ukrainian Biochemical Journal》 |2011年第6期|共7页
作者
L. G. Babich; S. G. Shlykov; N. V. Kandaurova; S. A. Kosterin;
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中图分类生物化学;
关键词
Ca2+flow cytometryisolated myometrium mitochondriamembrane potential;

机译：Ca2 +流式细胞仪分离的子宫肌层线粒体膜电位;

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2. Ca(2+)-dependent regulation of the Ca(2+) concentration in the myometrium mitochondria. I. Trifluoperazine effects on mitochondria membranes polarization and [Ca(2+)](m) [J] . L. G. Babich, S. G. Shlykov, A. M. Kushnarova, Ukrainian Biochemical Journal . 2016,第4期

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3. H~+-Ca~(2+) Exchanger in the Myometrium Mitochondria: Modulation by Exogenous and Endogenous Compounds [J] . O. V. Kolomiets, Yu. V. Danylovych, G. V. Danylovych International Journal of Physiology and Pathophysiology . 2015,第4期

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机译：热休克通过人类表皮样a 431细胞中的Na + -Ca sup 2 +交换增加细胞溶质游离Ca sup 2+浓度

Transmembrane Ca(2+) exchange in depolarized rat myometrium mitochondria

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