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首页> 外文期刊>Ukrainian Biochemical Journal >Proteins of astroglia in the rat brain under experimental chronic hepatitis and 2-oxoglutarate effect
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Proteins of astroglia in the rat brain under experimental chronic hepatitis and 2-oxoglutarate effect

机译:实验性慢性肝炎和2-氧戊二酸作用下大鼠大脑中星形胶质蛋白

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A probable increase of the level of calcium-binding protein S100b and soluble form of glial fibrillary acidic protein (GFAP), as well as reducing the level of filament GFAP in the brain of Wistar rats under hepatic encephalopathy development caused by chronic hepatitis (HP) were shown. Increasing concentrations of S100b may stimulate the disassembly of intermediate filaments of astrocytes. The immunohistochemical analysis helps to reveal that astrocytes in the brain of rats that had HP lose the characteristic stellate shape and swelling. Immunoblotting result have shown the fragmentation of the main filament form of GFAP and appearance of low mass derivates. Application of 2-oxoglutarate (2.28 g/l of drinking water during 10 days after the onset of chronic hepatitis) stabilized the studied proteins and the state of astroglia.
机译:慢性肝炎(HP)引起的肝性脑病发展后Wistar大鼠大脑中钙结合蛋白S100b和可溶性形式的胶质纤维酸性蛋白(GFAP)的水平可能增加,以及降低细丝GFAP的水平被显示。 S100b浓度的增加可能会刺激星形胶质细胞中间丝的分解。免疫组化分析有助于揭示患有HP的大鼠大脑中的星形胶质细胞失去特征性的星状形状和肿胀。免疫印迹结果表明,GFAP主要细丝形式的断裂和低质量衍生物的出现。施用2-氧戊二酸酯(在慢性肝炎发作后的10天内喝水2.28 g / l)稳定了所研究的蛋白质和星形胶质细胞的状态。

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