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首页> 外文期刊>Ukrainian Biochemical Journal >Liver cytochrome P450-hydroxylation system of tumor-bearing rats under the influence of ω-3 polyunsaturated fatty acids and vitamin D(3)
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Liver cytochrome P450-hydroxylation system of tumor-bearing rats under the influence of ω-3 polyunsaturated fatty acids and vitamin D(3)

机译：ω-3多不饱和脂肪酸和维生素D影响的荷瘤大鼠肝细胞色素P450羟化系统（3）

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The study was performed to investigate the effects of the separate and combined action of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) and vitamin Dsub3/sub on the activity of the components of the oxygenase and reductase chains of the monooxygenase system (MOS) in the microsomal fraction isolated from the liver of rats with transplanted Guerin carcinoma. In the liver of the tumor-bearing rats during the intensive growth of the tumor (14 days, which corresponds to the logarithmic phase of tumor growth), the functional activity of the MOS was weakened. N-demethylase, p-hydroxylase and NADPH-cytochrome P450 reductase activity decreased, with the simultaneous enhancement of cytochrome P450 inactivation rate due to its transformation into an inactive form, cytochrome P420. In turn, we found an increase in the functional activity of the reductase chain of MOS, which components are known to transfer electrons from the reduced NADH through NADH-cytochrome bsub5/sub-reductase and cytochrome bsub5/sub to cytochrome P450. In particular, the activity of NADH-cytochrome bsub5/sub-reductase and the rate of reduction of cytochrome bsub5/sub were elevated with a simultaneous decrease in its content. Both ω-3 PUFAs and vitamin Dsub3/sub administration to tumor-bearing rats for 42 days (28 days of preliminary administration and 14 days of tumor growth) significantly normalized the oxygenase activity of MOS, increasing NADPH-cytochrome P450-reductase, N-demethylase and p-hydroxylase activity of cytochrome P450 and blocking cytochrome P450 inactivation rate in the microsomal fraction of the liver. Administration of ω-3 PUFAs in combination with vitamin Dsub3/sub led to the synergy. Changes in the activity of the components of the reductase chain of MOS in liver of tumor-bearing rats were observed mainly after ω-3 PUFAs supplementation. The content of cytochrome bsub5/sub increased and the rate of its reduction was significantly diminished. In the absence of a pronounced individual effect of vitamin Dsub3/sub on the reductase chain of MOS, its co-administration with ω-3 PUFA was also found to be ineffective.
机译：进行了这项研究以研究omega-3多不饱和脂肪酸（ω-3PUFAs）和维生素D 3 的单独和联合作用对加氧酶和还原酶链的活性的影响鼠肝癌大鼠肝脏分离的微粒体部分中的单加氧酶系统（MOS）在肿瘤的密集生长期间（14天，对应于肿瘤生长的对数期），在荷瘤大鼠的肝脏中，MOS的功能活性减弱。 N-脱甲基酶，p-羟化酶和NADPH-细胞色素P450还原酶活性降低，同时由于细胞色素P450转化为非活性形式细胞色素P420而使其失活率同时提高。反过来，我们发现MOS还原酶链的功能活性增加，已知该组分可通过NADH-细胞色素b 5 -还原酶和细胞色素b 从还原的NADH转移电子。 5 至细胞色素P450。特别是，NADH-细胞色素b 5 -还原酶的活性和细胞色素b 5 的还原率均升高，同时含量降低。对荷瘤大鼠施用ω-3PUFA和维生素D 3 42天（初次施用28天，肿瘤生长14天）均显着使MOS的氧化酶活性正常化，从而增加了NADPH-细胞色素肝微粒体中细胞色素P450的P450还原酶，N-脱甲基酶和p-羟化酶活性以及阻断细胞色素P450的失活率。 ω-3PUFAs与维生素D 3 联合给药可产生协同作用。主要观察到补充ω-3PUFAs后，荷瘤大鼠肝脏中MOS还原酶链组成活性的变化。细胞色素b 5 的含量增加，其还原速率明显降低。在缺乏维生素D 3 对MOS还原酶链的明显作用的情况下，还发现其与ω-3PUFA的共同给药无效。

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