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首页> 外文期刊>Ukrainian Biochemical Journal >Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia
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Responsiveness to progesterone and potassium channel blockers 4-aminopyridine, tetraethylammonium and free Ca(2+) contentration in spermatozoa of patients with oligozoospermia/leucocytospermia

机译：对孕酮和钾通道阻滞剂4-氨基吡啶，四乙铵和游离Ca（2+）含量在少精子症/白细胞精子症患者精子中的反应

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摘要

The present study was undertaken to evaluate [Casup2+/sup]subi/sub signals that occur in human sperm cells exposed in vitro to three diverse compounds; progesterone, 4-aminopyridine (a highly effective inducer of hyperactivation in human sperm) and tetraethylammonium. The [Casup2+/sup]subi/sub reached after the extracellular calcium treatment was always higher in normozoospermic samples pretreated with progesterone than in pathozoospermic samples pretreated with progesterone. There were no changes in calcium signal in spermatozoa pretreated with progesterone from patients with oligozoospermia and leucocytospermia compared to control samples (without progesterone). [Casup2+/sup]subi/sub was always higher in pathozoospermic samples without 4-aminopyridine and always lower in pathozoospermic samples with 4-aminopyridine compared to these values in normozoospermic men. The 2 mM extracellular calcium administration to spermatozoa pretreated with tetraethylammonium did not result in a detectable increase in [Casup2+/sup]subi/sub in normo- and pathozoospermic samples. The mechanisms of progesterone-dependent activation of the Casup2+/sup-entry and the functioning of the voltage gated Casup2+/sup-channels of plasmalemma are disturbed in pathologies – there was no increase in the Casup2+/sup level in the conditions of Ksup+/sup-depolarization (in the presence of inhibitors of Ksup+/sup-channels).
机译：本研究旨在评估在体外暴露于三种不同化合物的人类精子细胞中发生的[Ca 2 + ] i 信号。孕酮，4-氨基吡啶（人精子过度活化的高效诱导剂）和四乙铵。孕酮预处理的正常精子样品中，胞外钙处理后达到的[Ca 2 + ] i 始终高于孕酮预处理的病态精子样品。与对照样品（不含孕酮）相比，经少精子症和白细胞精子症患者孕酮预处理的精子中钙信号没有变化。与正常精子症男性相比，[Ca 2 + ] i 在无4-氨基吡啶的病态精子样本中总是较高，而在具有4-氨基吡啶的病态精子样本中总是更低。用四乙铵预处理对精子进行2 mM的细胞外钙给药，在正常精子和病理精子样品中[Ca 2 + ] i 均未检测到增加。 Ca 2 + 条目的孕酮依赖性激活机制和质膜的电压门控Ca 2 + 通道的功能在病理学中受到干扰–没有发现在K + -去极化条件下（在存在K + -通道抑制剂的情况下），Ca 2 + 水平增加。

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