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首页> 外文期刊>Ukrainian Biochemical Journal >Identification of Tudor domain containing 7 protein as a novel partner and a substrate for ribosomal protein S6 kinaseS – S6K1 and S6K2
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Identification of Tudor domain containing 7 protein as a novel partner and a substrate for ribosomal protein S6 kinaseS – S6K1 and S6K2

机译：鉴定含有7种蛋白质的Tudor域作为新型伴侣和核糖体蛋白S6激酶S – S6K1和S6K2的底物

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Ribosomal protein S6 kinases (S6Ks) are principal regulators of cell size, growth and metabolism. Signaling via the PI3K/mTOR pathway mediates the activation of S6Ks in response to various mitogenic stimuli, nutrients and stresses. To date, the regulation and cellular functions of S6Ks are not fully understood. Our aim was to investigate and characterize the interaction of S6Ks with the novel binding partner of S6Ks, Tudor domain containing 7 protein (TDRD7), which is a scaffold protein detected in complexes involved in the regulation of cytoskeleton dynamics, mRNA transport and translation, non-coding piRNAs processing and transposons silencing. This interaction was initially detected in the yeast two-hybrid screening of HeLa cDNA library and further confirmed by pull-down and co-immunoprecipitation assays. In addition we demonstrated that TDRD7 can form a complex with other isoform of S6K – S6K2. Notably, both isoforms of S6K were found to phosphorylate TDRD7 in vitro at multiple phosphorylation sites. Altogether, these findings demonstrate that TDRD7 is a novel substrate of S6Ks, suggesting the involvement of S6K signaling in the regulation of TDRD7 cellular functions.
机译：核糖体蛋白S6激酶（S6Ks）是细胞大小，生长和代谢的主要调节剂。通过PI3K / mTOR途径发出的信号介导S6K的激活，以响应各种促有丝分裂的刺激，营养和压力。迄今为止，尚未完全了解S6K的调控和细胞功能。我们的目的是研究和表征S6Ks与S6Ks的新型结合伴侣-含有7个蛋白的Tudor域（TDRD7）之间的相互作用，该蛋白是在涉及调节细胞骨架动力学，mRNA转运和翻译的复合物中检测到的支架蛋白，编码的piRNA处理和转座子沉默。最初在酵母双杂交筛选HeLa cDNA文库中检测到了这种相互作用，并通过下拉法和免疫共沉淀法进一步证实了这种相互作用。此外，我们证明了TDRD7可以与S6K – S6K2的其他同工型形成复合物。值得注意的是，发现S6K的两个同工型在体外在多个磷酸化位点都使TDRD7磷酸化。总而言之，这些发现证明TDRD7是S6K的新型底物，表明S6K信号传导参与了TDRD7细胞功能的调节。

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