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首页> 外文期刊>Ukrainian Biochemical Journal >Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405
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Peculiarities of glucose and glycerol metabolism in Nocardia vaccinii IMB B-7405

机译：诺卡氏菌IMB B-7405中葡萄糖和甘油代谢的特殊性

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It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NADsup+/sup-dependent glucose-6- phosphate dehydrogenase and FADsup+/sup-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol?minsup-1/sup?mgsup-1/sup of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADPsup+/sup-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol?minsup-1/sup?mgsup-1/sup of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol?minsup-1/sup?mgsup-1/sup of protein) and through dihydroxyacetone. Replenishment of the Csub4/sub-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714–803 nmol?minsup-1/sup?mgsup-1/sup of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP- carboxykinase and PEP-synthase), trehalose phosphate synthase and NADPsup+/sup-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glyco- and aminolipids, respectively.
机译：已经确定在诺卡氏菌诺曼氏菌IMB B-7405（表面活性剂生产者）的细胞中，葡萄糖分解代谢是通过戊糖磷酸循环以及葡萄糖酸来进行的（NAD + 依赖性葡萄糖-6-磷酸的活性脱氢酶和依赖FAD + 的葡萄糖脱氢酶835±41和698±35 nmol？min -1 ？mg -1 。葡萄糖酸激酶反应中形成的6-磷酸葡萄糖酸参与了戊糖磷酸循环（组成型NADP + 依赖性的6-磷酸葡萄糖酸脱氢酶357±17 nmol？min -1 ？ mg -1 蛋白）。甘油分解为二羟基丙酮磷酸（糖酵解的中间体）的方法有两种：通过3-磷酸甘油酯（甘油激酶活性为244±12 nmol？min -1 ？mg -1 <蛋白质）并通过二羟基丙酮。在葡萄糖和甘油上生长的牛痘猪笼草IMV B-7405中C 4 -二羧酸池的补充发生在磷酸烯醇丙酮酸（PEP）羧化酶反应中（714-803 nmol？min -1 ？mg -1 蛋白）。 2-氧戊二酸通过2-oxoglutarate合酶的参与通过交替途径参与三羧酸循环。观察到的糖异生的两个关键酶（PEP-羧激酶和PEP合酶），海藻糖磷酸合酶和NADP + 依赖性谷氨酸脱氢酶的活性证实了IMV B-7405菌株对表面合成的能力。活性糖脂和氨基脂。

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