Modulation of LPS-induced ROS production and NF-κB nuclear translocation by N-stearoylethanolamine in macrophages

A. G. Berdyshev; H. V. Kosiakova; N. M. Hula

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Modulation of LPS-induced ROS production and NF-κB nuclear translocation by N-stearoylethanolamine in macrophages

机译：N-硬脂酰乙醇胺在巨噬细胞中对LPS诱导的ROS产生和NF-κB核转运的调节

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N-Stearoylethanolamine (NSE) is a minor lipid that belongs to the N-acylethanolamines family that mediates a wide range of biological processes. The effect of the NSE on reactive oxygen species (ROS) production and NF-κB activation stimulated by lipopolysaccharide (LPS) in rat peritoneal macrophages (PM) was evaluated. PM were obtained from the rat peritoneal cavity. ROS were detected following DCFDA and DHE fluorescence. Nuclear translocation of p65 NF-κB was examined by immunofluorescent method using confocal microscopy. It was shown that NSE exposure to peritoneal macrophages (10sup-7/sup M) prior to 30 min LPS stimulation inhibited super oxide and hydrogen peroxide production and NF-κB translocation into nuclei. Thus, NSE exhibits therapeutic potential to treat inflammatory diseases associated with increased activation of macrophages.

机译：N-硬脂酰乙醇胺（NSE）是一种较小的脂质，属于N-酰基乙醇胺家族，其介导广泛的生物过程。评估了NSE对大鼠腹膜巨噬细胞（PM）中脂多糖（LPS）刺激的活性氧（ROS）产生和NF-κB活化的影响。 PM从大鼠腹膜腔获得。 DCFDA和DHE荧光检测到ROS。使用共聚焦显微镜通过免疫荧光法检查了p65NF-κB的核易位。结果表明，在LPS刺激30分钟之前，NSE暴露于腹膜巨噬细胞（10 -7 M）会抑制过氧化物和过氧化氢的产生以及NF-κB移位进入细胞核。因此，NSE显示出治疗与巨噬细胞活化增加有关的炎性疾病的治疗潜力。

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《Ukrainian Biochemical Journal》 |2017年第5期|共8页
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A. G. Berdyshev; H. V. Kosiakova; N. M. Hula;
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中图分类生物化学;
关键词
LPSN-stearoylethanolaminereactive oxygen speciestranslocation of NF-κB in macrophages in rat;

机译：大鼠巨噬细胞中LPSN-硬脂酰乙醇胺活性氧的NF-κB迁移;

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1. Elevated pre-activation basal level of nuclear NF-κB in native macrophages accelerates LPS-induced translocation of cytosolic NF-κB into the cell nucleus [J] . Alexander V. Bagaev, Anastasiya Y. Garaeva, Ekaterina S. Lebedeva, Scientific reports. . 2019,第1期

机译：天然巨噬细胞核NF-κB的升高的前激活基础水平加速LPS诱导的细胞溶质NF-κB转移到细胞核中
2. Parthenolide inhibits the LPS-induced secretion of IL-6 and TNF-α and NF-κB nuclear translocation in BV-2 microglia [J] . Magni P., Ruscica M., Dozio E., Phytotherapy research: PTR . 2012,第9期

机译：车前草内酯抑制LPS诱导BV-2小胶质细胞分泌IL-6和TNF-α及NF-κB核易位
3. Extracellular polysaccharide from Bacillus sp. strain LBP32 prevents LPS-induced inflammation in RAW 264.7 macrophages by inhibiting NF-κB and MAPKs activation and ROS production [J] . DiaoY., XinY., ZhouY., International immunopharmacology . 2014,第1期

机译：来自芽孢杆菌属的细胞外多糖。 LBP32菌株通过抑制NF-κB和MAPKs活化以及ROS的产生来预防LPS诱导的RAW 264.7巨噬细胞炎症
4. EFFECT OF ANTI-TNF-o MAB ON NF-κB NUCLEAR TRANSLOCATION AND PROLIFERATION OF HUMAN HEPG2 CELLS [C] . Zhang Haijian, Yao Dengfu, Wu Wei, Progress on post-genome technologies and modern natural products . 2011

机译：抗TNF-o MAB对人HEPG2细胞NF-κB核转运和增殖的影响
5. Cyclic stretch force induces periodontal ligament cells to secrete exosomes that suppress IL-1β production through the inhibition of the NF-κB signaling pathway in macrophages [D] . WANG ZHUYU 2019

机译：循环拉伸力诱导牙周膜细胞分泌外来体，通过抑制巨噬细胞中的NF-κB信号通路抑制IL-1β的产生
6. Elevated pre-activation basal level of nuclear NF-κB in native macrophages accelerates LPS-induced translocation of cytosolic NF-κB into the cell nucleus [O] . Alexander V. Bagaev, Anastasiya Y. Garaeva, Ekaterina S. Lebedeva, -1

机译：天然巨噬细胞中核NF-κB的激活前基础水平升高加速了LPS诱导的胞浆NF-κB向细胞核的转运
7. Modulation of LPS-induced ROS production and NF-?B nuclear translocation by N-stearoylethanolamine in macrophages [O] . A. G. Berdyshev, H. V. Kosiakova, N. M. Hula 2017

机译：N-Stearoylethanolamine在巨噬细胞中的LPS诱导的ROS产生和NF-αb核易位的调节

Modulation of LPS-induced ROS production and NF-κB nuclear translocation by N-stearoylethanolamine in macrophages

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