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首页> 外文期刊>Ukrainian Biochemical Journal >The ROS-generating and antioxidant systems in the liver of rats treated with prednisolone and vitamin D(3)
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The ROS-generating and antioxidant systems in the liver of rats treated with prednisolone and vitamin D(3)

机译：泼尼松龙和维生素D（3）处理的大鼠肝脏中ROS产生和抗氧化系统

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The mechanisms of glucocorticoid-induced disturbances of liver function is currently not fully clarified. Vitamin Dsub3/sub was previously shown to play an important role in the regulation of impaired oxidative metabolism and detoxification function of the liver associated with the effects of hepatotoxic compounds. The study was undertaken to define the intensity of oxidative metabolism in the rat liver and survival of hepatocytes after prolonged prednisolone administration and to assess whether vitamin Dsub3/sub is capable to counter glucocorticoid-induced changes. It has been shown that prednisolone (0.5 mg per animal for 30 days) leads to 1.6-fold increase in the percentage of necrotic cells among isolated hepatocytes as compared with the control. The glucocorticoid-induced impairment of hepatocellular function was accompanied by enhanced generation of reactive oxygen species (ROS), accumulation of TBA-active products and carbonylated proteins but reduced levels of free SH-groups of low molecular weight compounds. It was demonstrated a decrease in the activities of key enzymes of antioxidant system (SOD, catalase, glutathione peroxidase), whereas the activities of pro-oxidant enzymes NAD(P)H-quinone oxidoreductase and semicarbazide-sensitive amine oxidase were shown to be increased. Vitamin Dsub3/sub (and to greater extent in combination with α-tocopherol) administration (100 IU) on the background of glucocorticoid therapy caused normalizing effects on the level of ROS formation, oxidative modification of biomolecules and activity of antioxidant enzymes resulting in better survival of hepatocytes. These data suggest a potential role of vitamin Dsub3/sub in the regulation of oxidative metabolism alterations related to hepatotoxic action of glucocorticoids.
机译：糖皮质激素引起的肝功能障碍的机制目前尚不完全清楚。先前已证明维生素D 3 在调节肝脏氧化代谢和排毒功能中起重要作用，与肝毒性化合物的作用有关。这项研究的目的是确定长时间泼尼松龙给药后大鼠肝脏中氧化代谢的强度和肝细胞的存活，并评估维生素D 3 是否能够抵抗糖皮质激素诱导的变化。已经显示，泼尼松龙（每只动物0.5mg，持续30天）与分离的肝细胞相比导致坏死细胞百分比增加1.6倍。糖皮质激素诱导的肝细胞功能损害伴随着活性氧（ROS）生成的增加，TBA活性产物和羰基化蛋白的积累，但低分子量化合物的游离SH基团水平降低。结果表明，抗氧化系统的关键酶（SOD，过氧化氢酶，谷胱甘肽过氧化物酶）的活性降低，而前氧化酶NAD（P）H-醌氧化还原酶和对氨基脲敏感的胺氧化酶的活性增加。 。在糖皮质激素治疗的背景下施用维生素D 3 （并在更大程度上与α-生育酚联用）（100 IU）对ROS形成水平，生物分子的氧化修饰和H.抗氧化酶导致肝细胞更好的存活。这些数据表明维生素D 3 在调节与糖皮质激素的肝毒性作用有关的氧化代谢改变中的潜在作用。

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