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Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

机译：用于电池片工程的温度响应型聚合物改性表面

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In the past two decades, as a novel approach for tissue engineering, cell sheet engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm), which is a well-known temperature-responsive polymer, has been grafted on tissue culture polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C, where the PIPAAm modified surface is hydrophobic, cells can adhere, spread on the surface and grow to confluence. By decreasing temperature to 20 °C, since the surface turns to hydrophilic, cells can detach themselves from the surface spontaneously and form an intact cell sheet with extracellular matrix. For obtaining a temperature-induced cell attachment and detachment, it is necessary to immobilize an ultra thin PIPAAm layer on the TCPS surfaces. This review focuses on the characteristics of PIAPAm modified surfaces exhibiting these intelligent properties. In addition, PIPAAm modified surfaces giving a rapid cell-sheet recovery has been further developed on the basis of the characteristic of the PIPAAm surface. The designs of temperature-responsive polymer layer have provided an enormous potential to fabricate clinically applicable regenerative medicine.
机译：在过去的二十年中，作为一种用于组织工程的新方法，我们的实验室提出了细胞片工程。聚（N-异丙基丙烯酰胺）（PIPAAm）是一种众所周知的温度响应性聚合物，它已通过电子束辐照聚合反应接枝到组织培养聚苯乙烯（TCPS）表面上。在37°C时，PIPAAm修饰的表面是疏水的，细胞可以粘附，扩散在表面上并生长至汇合。通过将温度降低至20°C，由于表面变为亲水性，细胞可以自发地从表面脱离，形成带有细胞外基质的完整细胞片。为了获得温度诱导的细胞附着和脱离，有必要将超薄的PIPAAm层固定在TCPS表面上。这篇综述着重于表现出这些智能特性的PIAPAm改性表面的特性。另外，基于PIPAAm表面的特性，进一步开发了提供快速细胞片回收的PIPAAm改性表面。温度响应性聚合物层的设计为制造临床上可应用的再生医学提供了巨大的潜力。

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