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Physical Properties of Polypeptide Electrospun Nanofiber Cell Culture Scaffolds on a Wettable Substrate

机译:可湿性基质上的多肽电纺纳米纤维细胞培养支架的物理性质

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Physical properties of poly(L-ornithine) (PLO), a polycation, poly(L-glutamic acid4-co-L-tyrosine) (PLEY), a polyanion, and electrospun fibers made of these polymers have been determined and compared. The polymers adopted random coil-like conformations in aqueous feedstocks at neutral pH and in dehydrated cast films and fibers on glass, and the fibers comprised numerous counterions, according to spectral analysis. Adsorption of model proteins and serum proteins onto hydrated and crosslinked fibers depended on the electrical charge of the proteins and the fibers. The surface charge density of the fibers will be comparable to, but less than, the charge density on the outer leaflet of the plasma membrane of usual eukaryotic cells. The present analysis thus advances understanding of cell behavior on electrospun fiber scaffolds, a topic of considerable current interest.
机译:聚(L-鸟氨酸)(PLO),聚阳离子,聚(L-谷氨酸 4 -co-L-酪氨酸)(PLEY),聚阴离子和由其制成的电纺纤维的物理性质已经确定并比较了聚合物。根据光谱分析,该聚合物在中性pH的水性原料中以及在玻璃上的脱水流延薄膜和纤维中采用无规卷曲状构象,并且纤维包含许多抗衡离子。模型蛋白和血清蛋白在水合交联纤维上的吸附取决于蛋白和纤维的电荷。纤维的表面电荷密度将与普通真核细胞的质膜外小叶上的电荷密度相当,但小于。因此,本分析提高了对电纺纤维支架上细胞行为的理解,这是当前引起人们极大关注的一个话题。

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