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首页> 外文期刊>Polymers >The Effect of Plasma Treated PLGA/MWCNTs-COOH Composite Nanofibers on Nerve Cell Behavior
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The Effect of Plasma Treated PLGA/MWCNTs-COOH Composite Nanofibers on Nerve Cell Behavior

机译：等离子体处理的PLGA / MWCNTs-COOH复合纳米纤维对神经细胞行为的影响

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Electrospun nanofibrous scaffolds which can mimic the architecture of the natural extracellular matrix (ECM) are potential candidates for peripheral nerve repair application. Multi-walled carbon nanotubes (MWCNTs) are used in peripheral nerve repair due to their ability to promote neurite extension and support neural network formation. In this study, surface-modified nanofibrous scaffolds composed of poly(lactic- co -glycolic acid) (PLGA) and various ratios of carboxyl-modified MWCNTs (MWCNTs-COOH) (PC0, PC2, PC4 and PC8) were fabricated by electrospinning. The effects of MWCNTs-COOH on the fibers?￠???? morphology, diameter distribution, mechanical properties and surface hydrophilicity were characterized by Scanning Electron Microscopy (SEM), ImageJ software, tensile testing and water contact angle. Furthermore, air plasma treatment was applied to improve the surface hydrophilicity of the scaffolds, and the optimal treatment condition was determined in terms of surface morphology, water contact angle and PC12 cell adhesion. Plasma treated nanofibers (p-PC0, p-PC2, p-PC4 and p-PC8) under optimal treatment conditions were used for further study. PC12 cell proliferation and differentiation were both improved by the addition of MWCNTs-COOH in scaffolds. Additionally, the proliferation and maturation of Schwann cells were enhanced on scaffolds containing MWCNTs-COOH. The neurite outgrowth of rat dorsal root ganglia (DRG) neurons was promoted on MWCNTs-COOH-containing scaffolds, and those cultured on p-PC8 scaffolds showed elongated neurites with a length up to 78.27 ????m after 3 days culture. Our results suggested that plasma treated nanofibers under appropriate conditions were able to improve cell attachment. They also demonstrated that plasma treated scaffolds containing MWCNTs-COOH, especially the p-PC8 nanofibrous scaffold could support the proliferation, differentiation, maturation and neurite extension of PC12 cells, Schwann cells and DRG neurons. Therefore, p-PC8 could be a potential candidate for peripheral nerve regeneration application.
机译：可以模拟天然细胞外基质（ECM）结构的电纺纳米纤维支架是周围神经修复应用的潜在候选者。多壁碳纳米管（MWCNT）由于具有促进神经突延伸和支持神经网络形成的能力而被用于周围神经修复。在这项研究中，通过电纺丝制备了由聚乳酸-乙醇酸（PLGA）和各种比例的羧基改性MWCNTs（MWCNTs-COOH）（PC0，PC2，PC4和PC8）组成的表面改性的纳米纤维支架。 MWCNTs-COOH对纤维的影响形态，直径分布，力学性能和表面亲水性通过扫描电子显微镜（SEM），ImageJ软件，拉伸试验和水接触角进行表征。此外，进行了空气等离子体处理以改善支架的表面亲水性，并根据表面形态，水接触角和PC12细胞粘附性确定了最佳处理条件。在最佳处理条件下使用等离子体处理的纳米纤维（p-PC0，p-PC2，p-PC4和p-PC8）进行进一步研究。通过在支架中添加MWCNTs-COOH可以改善PC12细胞的增殖和分化。另外，在含有MWCNTs-COOH的支架上，雪旺细胞的增殖和成熟得到增强。在培养三天后，在含有MWCNTs-COOH的支架上促进了大鼠背根神经节（DRG）神经元的神经突生长，在p-PC8支架上培养的神经节长出了最长为78.27μm的神经突。我们的结果表明，在适当条件下进行等离子体处理的纳米纤维能够改善细胞附着。他们还证明了用血浆处理的含有MWCNTs-COOH的支架，特别是p-PC8纳米纤维支架可以支持PC12细胞，施万细胞和DRG神经元的增殖，分化，成熟和神经突延伸。因此，p-PC8可能是周围神经再生应用的潜在候选者。

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