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首页> 外文期刊>Pneumonia. >The upper respiratory tract microbiome of hospitalised patients with community-acquired pneumonia of unknown aetiology: a pilot study
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The upper respiratory tract microbiome of hospitalised patients with community-acquired pneumonia of unknown aetiology: a pilot study

机译：住院病因不明的社区获得性肺炎住院患者的上呼吸道微生物组：一项初步研究

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The composition of the upper respiratory tract microbiome may play an important role in the development of lower respiratory tract infections. Here, we characterised the microbiome of the nasopharynx and oropharynx of hospitalised patients with community-acquired pneumonia (CAP) with unknown aetiology in an attempt to obtain insight into the aetiology of CAP. A random sample of 10 patients hospitalised with CAP previously enrolled in a separate clinical trial (ClinicalTrials.gov registry, Study ID: NCT01248715) in which a complete microbiological workup was not able to define an aetiology were analysed in this pilot study. This larger trial ( n = 1,221) enrolled patients from 9 adult hospitals in Louisville, Kentucky, USA. Nasopharyngeal and oropharyngeal swabs were obtained for metagenomic analysis. Polymerase chain reaction (PCR) for Streptococcus pneumoniae was performed in all patients. One patient had a distinct nasophararyngeal microbiome consisting largely of Haemophilus influenzae . This was the only patient with a negative PCR for S. pneumoniae in both nasophararyngeal and oropharyngeal specimens. Overall, substantial differences were found between nasophararyngeal and oropharyngeal microbiomes. The upper respiratory tract microbiome of only one patient suggested H. influenzae as a probable aetiology of CAP. Although this was a pilot study of only 10 patients, the presence of S. pneumoniae in the upper respiratory tract of the other 9 patients warrants further investigation.
机译：上呼吸道微生物组的组成可能在下呼吸道感染的发生中起重要作用。在这里，我们对病因不明的社区获得性肺炎（CAP）住院患者的鼻咽和口咽微生物组进行了特征分析，以期了解CAP的病因。在该试验研究中，随机抽取了10例接受CAP住院治疗的患者，该患者先前参加了一项单独的临床试验（ClinicalTrials.gov注册，研究ID：NCT01248715），在该研究中，无法通过完整的微生物学检查确定病因。这项较大的试验（n = 1,221）招募了来自美国肯塔基州路易斯维尔的9家成人医院的患者。获得鼻咽和口咽拭子用于宏基因组学分析。所有患者均进行了肺炎链球菌的聚合酶链反应（PCR）。一名患者有独特的鼻咽微生物组，主要由流感嗜血杆菌组成。这是唯一在鼻咽和口咽标本中肺炎链球菌PCR阴性的患者。总体而言，发现鼻咽和口咽微生物组之间存在实质性差异。仅一名患者的上呼吸道微生物组提示流感嗜血杆菌可能是CAP的病因。尽管这只是一项仅10例患者的初步研究，但其他9例患者的上呼吸道中是否存在肺炎链球菌值得进一步研究。

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