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首页> 外文期刊>The Indian journal of medical research >Nucleic acid amplification tests (NAATs) for gonorrhoea diagnosis in women: Experience of a tertiary care hospital in north India
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Nucleic acid amplification tests (NAATs) for gonorrhoea diagnosis in women: Experience of a tertiary care hospital in north India

机译：核酸扩增试验（NAAT）在女性淋病诊断中的应用：印度北部一家三级医院的经验

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Background & objectives: Gonorrhoea is among the most frequent of the estimated bacterial sexually transmitted infections (STIs) and has significant health implications in women. The use of nucleic acid amplification tests (NAATs) has been shown to provide enhanced diagnosis of gonorrhoea in female patients. However, it is recommended that an on-going assessment of the test assays should be performed to check for any probable sequence variation occurring in the targeted region. In this study, an in-house PCR targeting opa-gene of Neisseria gonorrhoeae was used in conjunction with 16S ribosomal PCR to determine the presence of gonorrhoea in female patients attending the tertiary care hospitals. Methods: Endocervical samples collected from 250 female patients with complaints of vaginal or cervical discharge or pain in lower abdomen were tested using opa and 16S ribosomal assay. The samples were also processed by conventional methods. Results: Of the 250 female patients included in the study, only one was positive by conventional methods (microscopy and culture) whereas 17 patients were found to be positive based on PCR results. Interpretation & conclusions: The clinical sensitivity of conventional methods for the detection of N. gonorrhoeae in female patients was low. The gonococcal detection rates increased when molecular method was used giving 16 additional positives. Studies should be done to find out other gene targets that may be used in the screening assays to detect the presence of gonorrhoea.
机译：背景与目标：淋病是估计的细菌性传播感染（STI）中最常见的一种，对妇女的健康产生重大影响。已经证明，使用核酸扩增测试（NAAT）可以增强女性患者淋病的诊断。但是，建议对测试方法进行持续评估，以检查目标区域中可能发生的序列变异。在这项研究中，将针对淋病奈瑟氏球菌不透明基因的内部PCR与16S核糖体PCR结合使用，以确定在三级医院就诊的女性患者中是否存在淋病。方法：使用opa和16S核糖体检测法对250例女性患者的阴道样本进行了检测，这些患者患有阴道或宫颈分泌物或小腹疼痛。样品也通过常规方法进行处理。结果：纳入研究的250名女性患者中，只有1例通过常规方法（显微镜和培养）呈阳性，而根据PCR结果发现17例呈阳性。解释与结论：女性患者常规检测淋病奈瑟菌的方法临床敏感性较低。当使用分子方法时，淋球菌检出率增加了16个阳性。应该进行研究以找出可用于筛选测定中检测淋病的其他基因靶标。

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