Comparative Hypoglycemic Effects of Cassia Glauca Lam. in Streptozotocin- Induced Diabetic Rats

Md. Salahuddin; Sunil S. Jalalpure

摘要

The aim of this study was to evaluate comparative hypoglycemic activity of aqueous extract of Cassia glauca leaf and bark. The various parameters studied included effect on fasting blood glucose levels of normoglycemic rats, oral glucose tolerance test and hypoglycemic activity in streptozotocin induced diabetic rats. On oral administration , aqueous extract showed statistically significant (P < 0.001) effect by reducing the effect of external glucose load in diabetic rats. In chronic model of treatment, the difference between the experimental (Cassia glauca bark extract) rats in lowering the fasting plasma glucose levels was statistically significant (P < 0.001) as compared in diabetic rats on day 21. These findings suggest the significant hypoglycemic potential of the Cassia glauca bark extracts in ameliorating the diabetic conditions in diabetic rats. No significant effects were found in the normal rats. Source of Support: The authors sincerely thank Dr. F. V. Manvi, Principal, KLES’s College of Pharmacy for providing the necessary facilities for this work. Introduction Diabetes is a serious illness with multiple complications and premature mortality, accounting for at least 10% of total health care expenditure in many countries (King et al., 1998) and is likely to remain a significant threat to public health in the years to come (Marx, 2002).The prevalence of diabetes for all age-groups worldwide is projected to rise from 171 million in 2000 to 366 million in 2030 (Amos et al., 1997). Despite the considerable progress in the understanding and management of diabetes, the disease and the complications related to it continue to increase (Tiwari and Madhusudana, 2002). The ethnopharmacological use of herbal remedies for the treatment of diabetes mellitus is an area of study ripe with potential as a starting point in the development of alternative inexpensive therapies for treating the disease (Marles and Farnsworth, 1995; Eddouks et al., 2002). Cassia glauca Lam is a glabrous tree belonging to family Caesalpiniacea, found throughout India, tropical Asia and Australia. The leaves are long linear, acute, curved in shape. The flower is yellow in colour and shorter than the leaves (Kirtikar and Basu, 1935). Phytochemical study of Cassia glauca stem has indicated the presence of chrysophenol, physcion, stearic acid, ?-sitosterol and ?-D glucoside (Hemlata and Kalidhar, 1994). In folk medicine bark and leaves of Cassia glauca are used for the treatment of diabetes and gonorrhea. The leaves are used to treat blennorrhagia (Kirtikar and Basu, 1935). Here we report hypoglycemic activity of the aqueous extract from leaf and bark of Cassia glauca, with a view to provide scientific evidence of modern lines. Materials And Methods Collection of plant materialThe leaves and bark of Cassia glauca was collected during November 2007 from the Western ghats, Karnataka, India. The plant was identified by Dr. Harsha Hegde, Research Officer, RMRC, ICMR, Belgaum, Karnataka, India. A voucher specimen (SA-01) has been kept in herbarium of Department of Pharmacognosy, KLES College of Pharmacy, Belgaum, Karnataka, India.Preparation of the test samples Cassia glauca leaves and bark was air dried in the shade and cut into small pieces. Hundred grams each of leaves and bark was extracted with 1000 ml of water by the method of hot extraction at 60oC for 6 h with occasional shaking and the extracts were filtered and filtrate were evaporated to dryness under vacuum to yield dry residue. Animals in the control group received only the vehicle (Normal saline 5 ml/kg, b.w.) (Jain, 1968; Latha and Pari, 2004; Aslan et al. 2007). AnimalsWistar rats (180-200 g) were obtained from animal house, Department of Livestock Production, Government Veterinary College, Hebbal, Bangalore, India. Rats were kept in groups of six in plastic cages at controlled temperature (25°C) and humidity (about 40%) with a 12 h light cycle beginning at 7 am. Rats were provided with standard di

机译：这项研究的目的是评估比较的决明子和树皮的水提取物的降血糖活性。研究的各种参数包括对正常血糖大鼠禁食血糖水平的影响，链脲佐菌素诱导的糖尿病大鼠的口服葡萄糖耐量试验和降血糖活性。口服给药时，水提取物通过降低糖尿病大鼠中外部葡萄糖负荷的作用显示出统计学显着（P <0.001）的作用。在慢性治疗模型中，实验（Cassia glauca树皮提取物）大鼠之间在降低空腹血浆葡萄糖水平方面的差异与第21天的糖尿病大鼠相比在统计学上具有显着性差异（P <0.001）。这些发现表明，糖尿病大鼠具有明显的降血糖潜力决明子树皮提取物可改善糖尿病大鼠的糖尿病状况。在正常大鼠中未发现明显的作用。支持的来源：作者衷心感谢KLES药学院的校长F. V. Manvi博士为这项工作提供了必要的设施。引言糖尿病是一种具有多种并发症和过早死亡的严重疾病，在许多国家中，糖尿病至少占总医疗保健支出的10％（King等，1998），并且在今后几年中，仍可能对公共健康构成重大威胁。全世界所有年龄组的糖尿病患病率预计将从2000年的1.71亿增加到2030年的3.66亿（Amos等，1997）。尽管在糖尿病的理解和管理上取得了长足的进步，但该疾病及其相关的并发症仍在继续增加（Tiwari和Madhusudana，2002）。草药在治疗糖尿病方面的民族药理学研究是一个成熟的研究领域，具有潜在的潜力，可以作为开发治疗该疾病的廉价廉价疗法的起点（Marles和Farnsworth，1995; Eddouks等，2002）。决明子是一棵无毛的树，隶属于Caesalpiniacea家族，遍布印度，亚洲热带地区和澳大利亚。叶子长线性，锐利，弯曲。花为黄色，比叶短（Kirtikar和Basu，1935年）。决明子茎的植物化学研究表明，存在苯三酚，physcion，硬脂酸，β-谷甾醇和β-D葡萄糖苷（Hemlata和Kalidhar，1994）。在民间医学中，决明子的树皮和叶子用于治疗糖尿病和淋病。叶子用于治疗出血性出血（Kirtikar和Basu，1935年）。在这里，我们报告了决明子的叶和树皮的水提取物的降血糖活性，以期为现代品系提供科学依据。材料和方法植物材料的收集决明子的叶和树皮是在2007年11月从印度卡纳塔克邦的西高止山脉收集的。该工厂由印度卡纳塔克邦Belgaum的ICMR RMRC研究官员Harsha Hegde博士鉴定。凭证样本（SA-01）已保存在印度卡纳塔克邦Belgaum KLES药学院的生药学部门的植物标本室中。将测试样品的制备决明子叶和树皮在阴凉处风干并切成小块。通过在60oC下热提取6小时并不时摇动的方法，用1000 ml的水提取百克叶子和树皮，偶尔摇动，将提取物过滤，滤液在真空下蒸发至干，得到干残留物。对照组中的动物仅接受赋形剂（生理盐水5 ml / kg，体重）（Jain，1968; Latha和Pari，2004; Aslan等，2007）。动物Wistar大鼠（180-200g）获自印度班加罗尔Hebbal政府兽医学院畜牧生产部动物舍。将大鼠分成六只一组放在塑料笼中，在可控制的温度（25°C）和湿度（大约40％）下进行，从上午7点开始进行12小时的光照循环。给大鼠提供标准饮食

Comparative Hypoglycemic Effects of Cassia Glauca Lam. in Streptozotocin- Induced Diabetic Rats

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