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首页> 外文期刊>The Internet Journal of Pharmacology >Screening for Antimicrobial Activity of Ageratum conyzoides L.: A Pharmaco-Microbiological Approach
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Screening for Antimicrobial Activity of Ageratum conyzoides L.: A Pharmaco-Microbiological Approach

机译：香叶香茅的抗菌活性筛选：药物-微生物学方法。

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Background: Ageratum conyzoides L. is shown to exhibit antimicrobial activity against resistant and multiple resistant strains of Staphylococcus aureus in vitro. The aims of this study were to determine which solvent would be the most suitable to extract the active constituents from the plant, and besides, to determine the antimicrobial properties of the plant extracts against strains of resistant and multiple resistant pathogenic microorganisms. Study design: 186g of flowers, 98g of leaves, 300g of flowers, 160g of roots and 200g of whole plant were separately and successively soxhlet extracted with 40 to 60% petroleum ether, dichloromethane and methanol. Water extracts were also prepared. These crude extracts were screened for antimicrobial activities against typed cultures of Staphylococcus aureus NCTC 6571, Methicillin Resistant Staphylococcus aureus NCTC 12493 and clinical isolates of resistant strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Results: The methanolic extract inhibited the growth of all the strains of Staphylococcus aureus with a zone size ranging from 26-28mm in diameter. However, it was weakly active against Escherichia coli and had no inhibitory activity at all against Pseudomonas aeruginosa. Water extracts and 40-60% Petroleum ether extracts did not show any antibacterial activity against the test microorganisms.Conclusion: The methanolic crude extracts were shown to demonstrate the antimicrobial properties hence 98% methanol was discovered to be the best solvent among the three. Introduction Microbial resistance, a world health hazard, is dramatically increasing 1,2 hence evaluation of natural products to find new, safe and effective active compounds to rotate or substitute with the invalidated ones is one of the scientific strategies to combat drug-resistant pathogens 3 . One of such plants of medicinal value is Ageratum conyzoides L. Its common name is goat weed 4 . In Ghana, the following local names are given to the plant: Twi: Guankuro, Adwowa-kuro; Ewe: Nyigbe (cow weed), Mima, (faeces weed: because of its foul smell); Ga: Ntumlumlu; Nzima: Ebuakulo; Krobo: Tokuyowi (he-goat's crown of head; because of its smell) (Dokosi, 1998) 5 .The plant is used in Southern Africa for the treatment of fresh wounds and in Central Africa, the leaf is used to aid the healing of burn wounds 6 . In Ghana, it is used for treating chronic sores, sore eyes and dysentery 7 . In East Africa, it is used as a styptic 8 . In Central Africa, it is used to treat pneumonia 9 . In Cameroon and Congo, the traditional use is to treat fever, rheumatism, headache and colic 10 . In Brazil, aqueous extracts of leaves or whole plants have been used to treat spasms, colds and fevers 11,12,13,14,15 .The purpose of this study is to determine the best solvent for the crude extract extraction and to investigate the antimicrobial properties of the plant against resistant and multiple resistant strains of selected microorganisms (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). Materials and methods Fresh whole plants of Ageratum conyzoides were collected randomly from Cape Coast in August, 2005. The taxonomic identity of this plant was determined by the Department of Botany, University of Cape Coast. The fresh plants were washed under running tap water, air dried for five (5) days and then homogenized to coarse powder. This was stored in air tight bottles. For aqueous extraction, 100g of air-dried powder was placed in distilled water and boiled for two hours and furthermore left to soak overnight. The resulting suspensions were filtered and evaporated to dryness at 60 ° C in vacuum.186g of flowers, 98g of leaves, 300g of flowers, 160g of roots and 200g of whole plant were separately and successively soxhlet extracted with 40 to 60% petroleum ether, dichloromethane and methanol. The vacuum dried extracts were reconstituted in the respective solvents used for the extraction. Water extracts w
机译：背景：香叶香菇显示出对金黄色葡萄球菌耐药和多重耐药菌株的体外抗菌活性。这项研究的目的是确定哪种溶剂最适合从植物中提取活性成分，此外，还要确定植物提取物对耐药和多重耐药病原微生物菌株的抗微生物特性。研究设计：分别用40至60％的石油醚，二氯甲烷和甲醇分别提取索氏提取物，分别提取186g花，98g叶子，300g花，160g根和200g全株。还制备了水提取物。筛选了这些粗提物对金黄色葡萄球菌NCTC 6571，耐甲氧西林金黄色葡萄球菌NCTC 12493的分型培养物以及金黄色葡萄球菌，铜绿假单胞菌和大肠杆菌耐药菌株的临床分离株的抗菌活性。结果：甲醇提取物抑制了所有金黄色葡萄球菌菌株的生长，其直径范围为26-28mm。但是，它对大肠杆菌的活性较弱，对铜绿假单胞菌完全没有抑制活性。水提取物和40-60％的石油醚提取物对测试微生物没有任何抗菌活性。结论：甲醇粗提取物显示出抗菌性能，因此发现98％的甲醇是三者中最好的溶剂。引言微生物耐药性（一种严重危害世界健康的物质）正急剧增加1,2，因此评估天然产物以寻找新的，安全有效的活性化合物进行旋转或用无效化合物替代是对抗耐药病原体的科学策略之一3 。这种具有药用价值的植物之一是香叶香ger。它的通用名是山羊杂草4。在加纳，该植物使用了以下本地名称：Twi：Guangkuro，Adwowa-kuro;母羊：Nyigbe（牛杂草），Mima（粪便杂草：由于其难闻的气味）; Ga：Ntumlumlu; Nzima：Ebuakulo; Krobo：Tokuyowi（山羊的头顶;由于它的气味）（Dokosi，1998年）5。该植物用于南部非洲，用于治疗新鲜伤口，在中部非洲，其叶子用于帮助伤口愈合。烧伤6。在加纳，它用于治疗慢性疮，眼痛和痢疾7。在东非，它被用作定型笔8。在中非，它被用来治疗肺炎9。在喀麦隆和刚果，传统用途是治疗发烧，风湿病，头痛和绞痛10。在巴西，叶片或整株植物的水提物已被用于治疗痉挛，感冒和发烧11,12,13,14,15。本研究的目的是确定提取粗提物的最佳溶剂并研究该植物对所选微生物（金黄色葡萄球菌，大肠杆菌和铜绿假单胞菌）的抗性和多重抗性菌株的抗微生物特性。材料和方法2005年8月从开普敦海岸随机采集新鲜的香叶香脂全株。该植物的分类学特征由开普敦大学植物学系确定。新鲜的植物在流动的自来水下洗涤，风干五（5）天，然后均质成粗粉。将其储存在气密瓶中。为了进行水提取，将100g风干的粉末置于蒸馏水中并煮沸2小时，然后再浸泡过夜。将得到的悬浮液过滤并在真空中于60°C蒸发至干。分别分离186g花，98g叶，300g花，160g根和200g全株，然后依次用40％至60％的石油醚进行索氏提取，二氯甲烷和甲醇。将真空干燥的提取物重构在用于提取的各个溶剂中。水提取物w

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