• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Internet Journal of Neurology >Histopathologic Diagnosis, Cell Cycle Parameters and Clinical Behavior of 90 Egyptian Brain Tumor Cases
【24h】

Histopathologic Diagnosis, Cell Cycle Parameters and Clinical Behavior of 90 Egyptian Brain Tumor Cases

机译：90例埃及脑肿瘤病例的组织病理学诊断，细胞周期参数和临床行为

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This work aims at assessment of factors contributing to cell proliferation in relation to histopathologic diagnosis and clinical outcome of 90 brain tumour cases from Egypt. Cases were taken prospectively from the Neurosurgery Department of Mansoura University Hospitals, Egypt. Their median age was 46 years and their sex included 42 (46.7%) males and 48 (53.3%) females. Of these cases, 14 cases (15.6%) had an age <20 years. Brain biopsy samples were processed for histopathologic examination in addition to flow cytometeryic analysis of DNA ploidy pattern, apoptosis, p53 and Bcl2 expressions. Meningeal tumors were most frequent (37.8%) followed by astrocytic tumors (26.7%), sellar tumors (12.2%) while the neuroblastic tumors were detected in 10% of cases. Females were more affected by meningiomas and pituitary adenomas whereas males were more affected by astrocytic tumors. Older cases were affected mostly by meningeal and astrocytic tumors while the younger ones were more affected by neuroblastic tumors. Malignant tumors showed significant increased levels of mutant p53 expression, S phase of both diploid and aneuoploid cells than benign ones (P<0.05). On follow up, most of the cases affected with meningeal tumors had become symptom free while recurrence and death were mostly observed in astrocytic tumors. Significant increased expression of mutant p53 was also observed among recurrent cases (p<0.05) than cases that become free of symptoms. These results shows that cell cycle markers in addition to histopathology can help in predicting prognosis of brain tumours with a potential impact on management plan. Introduction The most common disease process to affect brain cells is neoplasia or tumor formation resulting in tumor of glia (glioma), meningeal cells (meningiomas), Schwann cells (schwannomas) and immune system cells (lymphomas). In children, brain tumor is the second most common form of cancer, surpassed only by leukemia. Unfortunately, many brain tumors are currently incurable. The majority of adult gliomas, for instance, is resistant to therapy and often causes death within a few years. Meningiomas and schwannomas on the other hand, are generally benign and can be treated by surgical resection. However, the removal of benign tumors from deep regions of the brain may carry considerable risk to the patient, and therefore some meningiomas or schwannomas may not be curable (1). Most brain tumors occur in otherwise normal adults; that is, in people without a family history of brain tumors and without a history of exposure to an environmental toxin as smoking, or exposure to head injury, electric wires, and drugs including medications during pregnancy (2).Cells reproduce by doubling their constituents, followed by division. The sum of the cell activities that is essential for their reproduction is defined as the cell cycle(3). The cell cycle is composed of major phases. G1 phase is the time gap between the end of previous mitosis and start of DNA synthesis. It is the most variable period in the cycle. During this phase, the cell may enter in a resting state called G0 state. In some tissues that do not divide, such as nerve cells and skeletal muscles and those which rarely divide as lymphocytes, the DNA assay indicates that its bulk is in G1 period only thus showing diploid DNA content. S-phase is the most constant period of the cycle. It lasts about 7 hours. During this phase the DNA mass increases in size and amount till it reaches double the basic amount in G1 phase ending with tetraploid cell (4c). G2 phase is relatively of short period (4 hours) in which cells having double the amount of DNA (4c) become ready for division. M-phase or mitosis phase is the shortest phase of the cell cycle it takes nearly one hour. It is the final and microscopically visible stage of an underlying alteration that has occurred at molecular as well as biochemical levels. The essential features of this stage is the equal morphologic distribution o
机译：这项工作旨在评估与埃及的90例脑肿瘤病例的组织病理学诊断和临床结果相关的促细胞增殖的因素。病例均来自埃及曼苏拉大学医院神经外科。他们的中位年龄为46岁，性别包括42位男性（46.7％）和48位女性（53.3％）。在这些病例中，有14例（15.6％）的年龄小于20岁。除流式细胞术分析DNA倍性模式，凋亡，p53和Bcl2表达外，还对脑活检样品进行了组织病理学检查。脑膜肿瘤最常见（37.8％），其次是星形细胞肿瘤（26.7％），鞍状肿瘤（12.2％），而神经母细胞瘤则占10％。女性受脑膜瘤和垂体腺瘤的影响更大，而男性受星形胶质细胞瘤的影响更大。老年病例主要受脑膜和星形细胞肿瘤的影响，而年轻病例则受神经母细胞瘤的影响更大。恶性肿瘤的p53突变体表达，二倍体和非整倍体细胞的S期均显着高于良性（P <0.05）。在随访中，大多数受脑膜肿瘤影响的病例已无症状，而复发和死亡多数见于星形细胞肿瘤。与没有症状的病例相比，在复发病例中也观察到突变体p53的表达显着增加（p <0.05）。这些结果表明，除组织病理学外，细胞周期标志物还可以帮助预测脑肿瘤的预后，从而对管理计划产生潜在影响。简介影响脑细胞的最常见疾病过程是瘤形成或肿瘤形成，导致神经胶质瘤（神经胶质瘤），脑膜细胞（脑膜瘤），雪旺氏细胞（神经鞘瘤）和免疫系统细胞（淋巴瘤）。在儿童中，脑肿瘤是第二大最常见的癌症形式，仅白血病就超过了。不幸的是，许多脑肿瘤目前无法治愈。例如，大多数成人神经胶质瘤对治疗有抵抗力，并经常在几年内导致死亡。另一方面，脑膜瘤和神经鞘瘤通常是良性的，可以通过手术切除来治疗。但是，从脑深部切除良性肿瘤可能会给患者带来巨大风险，因此某些脑膜瘤或神经鞘瘤可能无法治愈（1）。大多数脑肿瘤发生在其他正常成年人中。也就是说，在没有脑瘤家族病史，没有因吸烟而暴露于环境毒素的史中，或者在怀孕期间没有暴露于头部受伤，电线和药物（2）的史中（2）。细胞通过将其成分加倍来繁殖，然后是除法。对其繁殖必不可少的细胞活性的总和定义为细胞周期（3）。细胞周期由主要阶段组成。 G1期是先前有丝分裂结束与DNA合成开始之间的时间间隔。这是周期中变化最大的时期。在此阶段，单元可能会进入称为G0状态的静止状态。在一些不分裂的组织中，例如神经细胞和骨骼肌以及很少分裂成淋巴细胞的组织中，DNA分析表明其体积仅在G1期，因此显示出二倍体DNA含量。 S相是周期的最恒定周期。持续约7个小时。在此阶段，DNA质量不断增加，直至达到G1期基本量的两倍，并终止于四倍体细胞（4c）。 G2期相对较短（4小时），其中具有两倍数量的DNA（4c）的细胞准备分裂。 M期或有丝分裂期是细胞周期最短的一个阶段，大约需要一个小时。这是在分子以及生化水平上发生的基础变化的最终且在显微镜下可见的阶段。此阶段的基本特征是形态分布均匀

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号