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首页> 外文期刊>The Internet Journal of Orthopedic Surgery >Stability And Effect Of Storage Of Blood On Hematofluorometer Readings Of Zinc Protoporphyrin
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Stability And Effect Of Storage Of Blood On Hematofluorometer Readings Of Zinc Protoporphyrin

机译：血液的稳定性及其对原卟啉锌血细胞荧光仪读数的影响

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Aim: To study the stability and effect of storage, at 4° C temperature in the refrigerator, on blood, on the Hematofluorometer readings of Zinc Protoporphyrin (ZPP) over time.Study: 48 randomly collected samples were tested for ZPP values using Hematofluorometers. Results were compared using paired t-test.Results: Data analysis showed that ZPP results were stable up to day 4 from the day of blood collection. Comments: one should establish their own controls to find the stability of the results according to the locally prevailing conditions for the more reliable results in their practice. Introduction Hematofluorometric determination of Zinc Protoporphyrin (ZPP) is a screening method for the assessment of iron deficiency. Beyond that, ZPP is not only a simple parameter of detecting iron deficiency, but rather a parameter of iron deficient erythropoeisis surveying all the steps of iron metabolism. Therefore, ZPP is used as a kind of end point control to detect real iron deficiency as well as derangements of iron metabolism in chronic inflammatory disorders, neoplastic disorders, sideroblastic disorders or lead poisoning. [1]Hematofluorometer measures ZPP and a variable proportion of metal-free Protoporphyrin IX (PPN), depending upon the emission and excitation filters of the particular instrument. [2, 3] Only a small drop of blood is required for the test. The measurement takes less than one minute. Its being a simple test, we searched the literature to know the stability of the test and we could only find very few references. Therefore, we decided to study the stability and effect of storage of blood (at 4° C temperature in the refrigerator) on ZPP values over time in our set-up. Materials and Methods The study was carried out in a tertiary care hospital in the western region of India. 48 samples collected in 3 ml EDTA vacutainer, were selected randomly from the samples sent to our laboratory for various investigations, without any criteria for inclusion or exclusion to represent the whole strata of patients. Samples were tested for ZPP levels using Hematofluorometer (Protofluor-Z, Helena Laboratories). Each sample was subjected to ZPP estimation on the day of collection and then every day till one week from the day of sample collection. Tests were performed at the same time of the day throughout one week. Samples were preserved at the 4° C temperature in the refrigerator. Company provided reagents and calibrators were used for the test performance and calibration, respectively. Tests were performed at the room temperature. Samples and calibrators were allowed to come to room temperature before performing the tests. Results Results of the samples of the tests performed daily were analyzed using paired t-test. The ZPP value of day 1 of each sample was compared with that of the respective sample for each subsequent day. The p values of <0.05 were considered significant, meaning thereby that the readings of that particular day were significantly different from those of day 1 results and that implied the instability of the results then onwards. In our study, we observed that ZPP results were stable till day 4 (Table-1). Therefore, it is possible to reproduce and/or get reliable test results till day 4 from the day of collection of the blood under the circumstances prevailing in our laboratory.
机译：目的：研究冰箱在4°C温度下的储存稳定性和对血液的影响，以及随时间变化的原卟啉锌（ZPP）血液荧光计读数的研究。研究：使用血液荧光计测试48个随机收集的样品的ZPP值。结果采用配对t检验进行比较。结果：数据分析表明，ZPP结果从采血之日起第4天一直稳定。评论：人们应该建立自己的控制措施，以根据当地普遍的条件找到结果的稳定性，以便在实践中获得更可靠的结果。简介血液荧光法测定原卟啉锌（ZPP）是评估铁缺乏症的一种筛选方法。除此之外，ZPP不仅是检测铁缺乏症的简单参数，而且还是对铁代谢的所有步骤进行检测的铁缺乏症红细胞生成的参数。因此，ZPP被用作一种终点控制，以检测慢性炎性疾病，赘生性疾病，铁粒母疾病或铅中毒中的真正铁缺乏症以及铁代谢异常。 [1]根据特定仪器的发射滤光片和激发滤光片，血液荧光计可测量ZPP和可变比例的无金属原卟啉IX（PPN）。 [2，3]只需少量滴血即可进行测试。测量时间不到一分钟。它是一个简单的测试，我们搜索了文献以了解该测试的稳定性，并且我们只能找到很少的参考文献。因此，我们决定在我们的设置中研究血液储存（在冰箱中4°C温度下）随时间变化的ZPP值的稳定性和影响。材料和方法这项研究是在印度西部地区的一家三级医院进行的。从3毫升EDTA真空容器中收集的48个样本是从送往我们实验室进行各种调查的样本中随机选择的，没有任何纳入或排除标准来代表患者的整个阶层。使用血细胞荧光仪（Protofluor-Z，Helena Laboratories）测试样品的ZPP水平。每个样本在收集之日进行ZPP估算，然后每天直到样本收集之日起一周。在整个一周的一天中的同一时间进行测试。将样品保存在冰箱中的4°C温度下。公司提供的试剂和校准品分别用于测试性能和校准。测试在室温下进行。在进行测试之前，允许样品和校准物达到室温。结果使用配对t检验分析每天进行的测试的样品的结果。将每个样品的第一天的ZPP值与随后每一天的相应样品的ZPP值进行比较。 p值<0.05被认为是重要的，这意味着该天的读数与第1天的结果显着不同，这意味着以后的结果是不稳定的。在我们的研究中，我们观察到ZPP结果一直稳定到第4天（表1）。因此，在我们实验室普遍存在的情况下，从采血之日起第4天之前，有可能再现和/或获得可靠的测试结果。

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