...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Internet Journal of Infectious Diseases >Abrogation of attachment of Infectious Bursal Disease Virus to chicken B cells by treatment of B cells with Soyabean Agglutinin and Wheat Germ Lectin
【24h】

Abrogation of attachment of Infectious Bursal Disease Virus to chicken B cells by treatment of B cells with Soyabean Agglutinin and Wheat Germ Lectin

机译：通过用大豆凝集素和小麦胚芽凝集素处理鸡胚B细胞来避免鸡传染性法氏囊病病毒的附着

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Infectious Bursal Disease (IBD) is a viral disease of young chickens with high morbidity and low mortality. The target molecule on B cells for binding of IBD virus (IBDV) is not known. In this study, bursal B cells from chicken aged 6 weeks were treated with either Soyabean Agglutinin (SBA) or Wheat Germ Lectin (WGL) prior to incubation with IBDV. The mean percentages of B cells positive for IBDV binding without and after treatment of B cells with lectins SBA and WGL were 94.48 + 0.47, 88.52 + 0.54 and 88.86 + 0.35, respectively. The differences between the values without and after treatment in case of both the lectins were found to be highly significant (p<0.0001). However, the two lectins did not differ significantly with each other in their effects. ANOVA among the values of the three groups revealed very significant differences (F = 52.778; p = 0.0005) among them. The results suggest that the putative IBDV binding site on B cells may possibly be a lectin - binding molecule. Introduction Infectious Bursal Disease (IBD) is a major immunosuppressive disease of chickens having serious economic impact on the poultry industry worldwide. It is an acute, highly contagious disease of young chickens of 3 – 6 weeks of age caused by the Infectious Bursal Disease Virus (IBDV) belonging to the genus Avibirnavirus of family Birnaviridae (Dobos et al., 1979; Murphy et al., 1999). IBDV infection causes depletion of B cells by apoptosis (Jungmann et al., 2001). Although sIgM – bearing pre-B cells have been shown to be vulnerable for IBDV infection (Hirai and Calnek, 1979), the exact identity of the target receptor for IBDV attachment on pre-B cells is not known. If the specific molecule which serves as the target for IBDV attachment to B cells, could be identified, it will pave the way for effective control of IBD by blocking the interaction of IBDV with its ligand on B cell surface through immunological means or recombinant target protein. The present study was, therefore, undertaken to investigate the possible affinity of IBDV towards certain lectin – binding molecules on bursal B cells. Materials and methods The guidelines of the Institutional Animal Ethics Committee were followed in all the experiments. Experimental birds: Day – old White Leghorn male chicks were procured from the Hatchery of the Department of Animal Breeding and Genetics, PAU, Ludhiana and reared in the Animal House of the Department of Veterinary Microbiology till used at the age of 6 weeks. Virus preparation: The intermediate D-78 vaccine strain of IBDV was propagated in BGM-70 cells. Cell culture for propagation of virus: The BGM-70 cell line was gifted by Dr. Y. M. Saif and Dr. Robert Dearth, Ohio State University, Ohio, USA. The cells were cultured in Eagle's Minimum Essential Medium. The cells were grown to confluence in tissue culture flasks. The growth medium was removed and the cell monolayer was washed twice with the maintenance medium. Virus stock in maintenance medium (0.2 ml) was inoculated in the culture at 370C and monitored daily for cytopathic effects upto 5 days. At 5 days post-inoculation, the monolayer was disrupted by repeated freeze – thaw cycles and the suspension was clarified by low speed centrifugation. The supernatant fluid was harvested and the cell culture lysate was used as virus inoculum in the subsequent passage. The monolayer was infected and the second passaged virus / cell lysate was harvested, aliquoted and stored at –200C until used further. Collection of bursae from chicks: Healthy young chicks aged 6 weeks were slaughtered by cervical dislocation. Bursae were then immediately excised from the dead birds and collected in Hank's Balanced Salt Solution (HBSS). Preparation of bursal cells: Immediately after collection, each bursa was thoroughly rinsed in HBSS, dissected free of fat and capsule and transferred to fresh HBSS. After removing capsule it was finely chopped with scissors. Minced tissue was gently forced through 60 m pore si
机译：传染性法氏囊病（IBD）是一种发病率高，死亡率低的雏鸡的病毒性疾病。 B细胞上用于结合IBD病毒（IBDV）的靶分子是未知的。在这项研究中，在与IBDV孵育之前，将大豆凝集素（SBA）或小麦胚芽凝集素（WGL）处理了6周龄鸡的法氏囊B细胞。在未用凝集素SBA和WGL处理B细胞的情况下和之后用IBDV结合阳性的B细胞的平均百分比分别为94.48±0.47、88.52±0.54和88.86±0.35。发现在两种凝集素的情况下，未处理和处理后的值之间的差异非常显着（p <0.0001）。但是，两种凝集素的作用没有明显差异。三组值之间的方差分析显示它们之间存在非常显着的差异（F = 52.778; p = 0.0005）。结果表明，在B细胞上推定的IBDV结合位点可能是凝集素结合分子。简介鸡传染性法氏囊病（IBD）是一种主要的免疫抑制疾病，对全世界的家禽业产生严重的经济影响。这是一种由感染性法氏囊病病毒（BiDVaviridae属）感染的法氏囊病病毒（IBDV）引起的3-6周龄小鸡的急性，高度传染性疾病（Dobos等，1979; Murphy等，1999）。 ）。 IBDV感染通过凋亡导致B细胞耗竭（Jungmann等，2001）。尽管已显示携带sIgM的pre-B细胞易受IBDV感染（Hirai和Calnek，1979年），但尚不清楚IBDV附着在pre-B细胞上的靶受体的确切身份。如果能够鉴定出作为IBDV附着于B细胞的靶标的特定分子，它将通过免疫学手段或重组靶蛋白阻断IBDV与B细胞表面配体的相互作用，为有效控制IBD铺平道路。 。因此，本研究旨在研究IBDV对法氏囊B细胞上某些凝集素结合分子的可能亲和力。材料和方法所有实验均遵循机构动物伦理委员会的指导。实验鸟：从卢迪亚纳州动物保护协会动物繁殖和遗传学系孵化场购买白日长角horn雄性雏鸡，并在兽医微生物学系动物舍饲养，直到6周龄时使用。病毒制备：IBDV的中间D-78疫苗株在BGM-70细胞中繁殖。用于病毒繁殖的细胞培养：BGM-70细胞系由美国俄亥俄州立大学的Y. M. Saif博士和Robert Dearth博士提供。细胞在Eagle的最低必需培养基中培养。细胞在组织培养瓶中生长至汇合。除去生长培养基，并用维持培养基将细胞单层洗涤两次。在370℃的培养物中接种维持培养基（0.2ml）中的病毒原液，每天监测直至5天的细胞病变作用。接种后5天，单层被反复的冻融循环破坏，悬浮液通过低速离心来澄清。收集上清液，并在随后的传代中将细胞培养物裂解物用作病毒接种物。单层被感染，第二次传代的病毒/细胞裂解液被收集，等分并保存在–200C直至进一步使用。从雏鸡中收集法氏囊：通过颈脱位法杀死6周龄的健康幼雏。然后立即从死鸟身上摘下法氏囊，并收集在汉克的平衡盐溶液（HBSS）中。法氏囊细胞的制备：收集后，立即将每个法氏囊在HBSS中彻底冲洗，切掉脂肪和胶囊，然后转移到新鲜的HBSS中。取出胶囊后，用剪刀将其切碎。将切碎的组织轻轻推过60 m的孔

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号