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外文期刊>Protistology: An International Journal
>Light-microscopic morphology and ultrastructure of Polychaos annulatum (Penard, 1902) Smirnov et Goodkov, 1998 (Amoebozoa, Tubulinea, Euamoebida), re-isolated from the surroundings of St. Petersburg (Russia)
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Light-microscopic morphology and ultrastructure of Polychaos annulatum (Penard, 1902) Smirnov et Goodkov, 1998 (Amoebozoa, Tubulinea, Euamoebida), re-isolated from the surroundings of St. Petersburg (Russia)
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机译：从圣彼得堡（俄罗斯）周围重新隔离的无花果的光学显微镜形态和超微结构（Penard，1902）Smirnov et Goodkov，1998（Amoebozoa，Tubulinea，Euamoebida），1998年
We isolated the species Polychaos annulatum (Penard, 1902) Smirnov et Goodkov,1998 from a freshwater habitat in the surrounding of Saint-Petersburg. The previousre-isolation of this species took place in 1998; at that time the studies of its lightmicroscopicmorphology were limited with the phase contrast optics, and the electronmicroscopicdata were obtained using the traditional glutaraldehyde fixation, precededwith prefixation and followed by postfixation with osmium tetroxide. In the presentpaper, we provide modern DIC images of P. annulatum. Using the fixation protocolthat includes a mixture of the glutaraldehyde and paraformaldehyde we were ableto obtain better fixation quality for this species. We provide some novel details of itslocomotive morphology, nuclear morphology, and ultrastructure. The present findingevidence that P. annulatum is a widely distributed species that could be isolated froma variety of freshwater habitats.
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机译：我们从圣彼得堡周围的淡水生境中分离出了Polychaos annulatum（Penard，1902）Smirnov et Goodkov，1998。该物种先前的重新隔离发生在1998年;当时其光学显微形态的研究受到相衬光学器件的限制，并且使用传统的戊二醛固定，先加前缀，再用四氧化os后固定来获得电子显微数据。在本文中，我们提供了现代的P. annulatum DIC图像。使用包含戊二醛和多聚甲醛的混合物的固定方案，我们能够为该物种获得更好的固定质量。我们提供了其机车形态，核形态和超微结构的一些新颖细节。目前的发现表明，环孢假单胞菌是一种广泛分布的物种，可以从各种淡水生境中分离出来。
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