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首页> 外文期刊>The Journal of General and Applied Microbiology >A multipathogen selective enrichment broth for simultaneous growth of Salmonella spp., Vibrio parahaemolyticus, and Vibrio cholerae
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A multipathogen selective enrichment broth for simultaneous growth of Salmonella spp., Vibrio parahaemolyticus, and Vibrio cholerae

机译：一种多病原体选择性富集肉汤，用于沙门氏菌，副溶血性弧菌和霍乱弧菌的同时生长

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A selective enrichment broth (SVV) was formulated to allow concurrent growth of Salmonella spp., V. parahaemolyticus, and V. cholerae. Potassium tellurite and sodium citrate were added as the inhibitors, while glucose, mannitol, anhydrous sodium sulfite and sodium pyruvate were employed as the growth-promoters. When mixed in equal or varied proportions, the target pathogens in SVV had a great accumulation (105-108 CFU/ml) and effectively inhibited the growth of competitive microflora. In the artificially contaminated samples, a high recovery of these 3 target pathogens was obtained in SVV. Finally, Salmonella spp., V. parahaemolyticus, and V. cholerae were detected from 608 suspicious food samples by SVV with real-time PCR, and no false-positive or -negative results were reported. In summary, SVV has been shown to be a suitable broth for the simultaneous detection of the 3 pathogens by multipathogen detection on a single-assay platform.
机译：配制了一种选择性富集肉汤（SVV），以允许沙门氏菌，副溶血性弧菌和霍乱弧菌同时生长。加入碲酸钾和柠檬酸钠作为抑制剂，而葡萄糖，甘露醇，无水亚硫酸钠和丙酮酸钠用作生长促进剂。当以相等或不同比例混合时，SVV中的目标病原体具有大量积累（105-108 CFU / ml），并有效抑制竞争性菌群的生长。在人工污染的样品中，SVV获得了这3种目标病原体的高回收率。最终，通过实时PCR SVV从608个可疑食物样品中检测到沙门氏菌，副溶血性弧菌和霍乱弧菌，未报告假阳性或阴性结果。综上所述，SVV已被证明是一种适合在单一测定平台上通过多病原体检测同时检测3种病原体的肉汤。

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