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首页> 外文期刊>The Journal of Musculoskeletal and Neuronal Interactions >The effects of hypothyroidism on nerve growth factor and norepinephrine concentrations in weight-bearing and non-weight-bearing bones of rats
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The effects of hypothyroidism on nerve growth factor and norepinephrine concentrations in weight-bearing and non-weight-bearing bones of rats

机译：甲状腺功能减退对大鼠负重和负重骨骼神经生长因子和去甲肾上腺素浓度的影响

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Thyroid hormones affect bone remodelling directly via receptors in osteoblasts. Previously, however, we have shown that the euthyroid and hyperthyroid states significantly influence the concentrations of both nerve growth factor (NGF) and norepinephrine (NE) in particular bones. Both NGF and NE directly affect bone metabolism and therefore it is possible that thyroid hormone action on bone may also be indirect via its actions on these two neural-related substances. In light of previous studies, the current experiments aimed to investigate whether hypothyroidism also influenced NGF and NE concentrations in weight-bearing and nonweight- bearing rat bones. Hypothyroidism was induced by oral ingestion of propylthiouricil (PTU; 3.8 ± 0.2 mg/kg/day) for 21 days. Histological examination on distal femurs and microparticle enzyme immunoassayed plasma concentrations of T3 and T4 verified the hypothyroid status in treated rats. NGF concentrations were assayed via enzyme-linked immunosorbent assay (ELISA) and NE concentrations were measured via high performance liquid chromatography (HPLC) with electrochemical detection (ECD). NGF concentrations: Femoral NGF concentrations were 207% higher in hypothyroid rats (674.9 ± 88.3 ng/g) than in euthyroid rats (326.7 ± 63.6 ng/g; p < 0.05). Rib NGF concentrations in hypothyroid rats (3125.1 ± 450.2 ng/g) were increased by 342% compared to euthyroid ribs (914.5 ± 128.6 ng/g; p < 0.01). Rib NGF concentrations in hypothyroid rats were 463% higher than in femurs of hypothyroid rats (p < 0.001). NE concentrations: In hypothyroid rats, NE concentrations were reduced by approximately 50% in both ribs (38.9 ng/g) and calvaria (41.5 ng/g) compared to euthyroid rats (74.7 ng/g and 87.4 ng/g respectively; p < 0.05 for both). These findings on hypothyroid rats may be taken in conjunction with our companion work on hyperthyroid rats (Yao et al., 2002, JMNI 2:327-334) and put in context with other reports, to indicate that (i) there are several sources of NGF in bone, some of which are stimulated by hypothyroidism and others by hyperthyroidism and (ii) the concentrations of both NGF and NE in bone are sensitive to weight-bearing and thyroid hormone status
机译：甲状腺激素直接通过成骨细胞中的受体影响骨骼重塑。但是，以前我们已经证明，正常甲状腺和甲状腺功能亢进状态会显着影响特定骨骼中神经生长因子（NGF）和去甲肾上腺素（NE）的浓度。 NGF和NE都直接影响骨骼代谢，因此甲状腺激素对骨骼的作用也可能通过其对这两种神经相关物质的作用而间接作用。根据先前的研究，当前的实验旨在研究甲状腺功能减退是否也影响了负重和不负重大鼠骨骼中NGF和NE的浓度。口服甲硫尿嘧啶（PTU; 3.8±0.2 mg / kg /天）持续21天可诱发甲状腺功能减退症。股骨远端组织学检查和微粒酶免疫测定的血浆T3和T4浓度证实了所治疗大鼠的甲状腺功能减退状态。 NGF浓度通过酶联免疫吸附测定（ELISA）进行测定，NE浓度通过高效液相色谱（HPLC）和电化学检测（ECD）进行测定。 NGF浓度：甲状腺功能减退大鼠（674.9±88.3 ng / g）的股骨NGF浓度高于正常甲状腺大鼠（326.7±63.6 ng / g; p <0.05）。与正常甲状腺的肋骨（914.5±128.6 ng / g）相比，甲状腺功能减退大鼠的肋骨NGF浓度（3125.1±450.2 ng / g）增加了342％。甲状腺功能减退大鼠的肋骨NGF浓度比甲状腺功能减退大鼠的股骨高463％（p <0.001）。 NE浓度：在甲状腺功能减退的大鼠中，与正常甲状腺的大鼠（分别为74.7 ng / g和87.4 ng / g）相比，肋骨（38.9 ng / g）和颅骨（41.5 ng / g）的NE浓度均降低了约50％。两者均为0.05）。这些关于甲状腺功能低下大鼠的发现可能与我们对甲状腺功能亢进的大鼠的伴随研究相结合（Yao et al。，2002，JMNI 2：327-334），并与其他报告结合起来，以表明（i）有多种来源骨中的NGF含量，其中一些是由甲状腺功能减退刺激的，而其他则是由甲状腺功能亢进刺激的;（ii）骨中NGF和NE的浓度均对体重和甲状腺激素状态敏感

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