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首页> 外文期刊>The Internet Journal of Tropical Medicine >Hepatoprotective and antioxidant effect of Sphaeranthus indicus against acetaminophen–induced hepatotoxicity in rats.
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Hepatoprotective and antioxidant effect of Sphaeranthus indicus against acetaminophen–induced hepatotoxicity in rats.

机译：球形菊抗对乙酰氨基酚引起的大鼠肝毒性的肝保护和抗氧化作用。

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The flower heads of Sphaeranthus indicus Linn (Asteraceae) a traditional Indian medicinal plant is commonly used to nourish and improve the liver conditions. This study was designed to evaluate the hepatoprotective and antioxidant effect of aqueous (AQS) and methanolic (MES) extract of flower heads of Sphaeranthus indicus on Paracetamol (APAP)-induced hepatotoxicity in rat’s in-vivo. Activities of liver marker enzymes ,glutamate-oxaloacetate transaminase (SGOT) glutamate pyruvate transaminase (SGPT), acid phosphatase (ACP) and alkaline phosphatase (ALP) bilirubin and total protein at an oral dose of MES (300mg/kg) showed a significant hepatoprotective effect in comparison with the same dose of aqueous extract. This fact was also confirmed by studying the liver histopathology of treated animals. As Regards the antioxidant activity, MES exhibited a significant effect (P < 0.05) showing increasing levels of superoxide dismutase (SOD), Catalase (CAT), and glutathione peroxides (GPX) by reducing malondialdehyde (MDA) levels. Introduction The pharmaceutical imbalance between remedies that protect the liver and have antioxidant properties and drugs that induce hepatotoxicity has prompted and accelerated research into plants used in folk medicines to treat liver diseases and boost liver functions. Sphaeranthus indicus Linn. (Asteraceae) commonly in Hindi known, as Gorakhmundi is an annual spreading herb, distributed through out the plains and wetlands of India, Sri Lanka and Australia.1, 2 The entire plant is reportedly used in the ayurvedic system of medicines in the treatment of epilepsy and mental disorders.3 It reportedly used to cure piles, hepatitis.4 and have protection against immunosupression.5 Literature reports on the ariel parts of this plant revealed the presence of an essential oil glucosides, and eudesmanoids.6an alkaloid sphaeranthine and an isoflavone 5,4‘-dimethoxy-3‘-prenylbiochanin 7-o-β-galctoside with some interesting sesquiterpene.8,9,10 and a new flavone glycoside.11 from the stem have been isolated from this herb. In this study, we have investigated the ability of flower heads extracts of Sphaeranthus indicus to protect liver against paracetamol induced hepatocellular damage and oxidative stress in rats in-vivo. Materials And Methods Plant materials: Dried flower heads of Sphaeranthus indicus (SI) were procured from local drug market of Meerut U.P, India and were identified by Dr. H.B Singh, National Institute of Science Communication and Information Resources (NISCAIR) New Delhi, India. A voucher specimen was deposited at the herbarium of our Pharmacognosy laboratory.Extraction: Dried powdered flower heads were powdered mechanically (Sieve No. 10/44) about 250g of the powder was thougherly extracted with methanol in a soxhlet apparatus. Another 250g of powdered material was completely extracted in boiling distilled water. A rotary vacuum evaporator concentrated both the extracts the percentage yield of both the extracts 22.5% and 20.9% respectively. The residue of both extracts made into a suspension in water and propylene glycol (4:1) containing Tween-80 (0.08%) at the concentration of 200mg/ml separately. Animals: Thirty pathogen free male albino rats (four weak) of either sex weighing 180-200g respectively were used for the study. They were housed in specific standard laboratory conditions and were kept in temperature control environment (25±10C), in a relative humidity (55± 5%), with regular 12h light/12h dark cycle. All animals were fed with standard rat chow diet, water ad libitum and received humane care in accordance approval of Institutional ethics committee rules.Hepatoprotective activity: Rats were divided into five groups, with six animals in each group. Group I, the normal control group animals were administered p.o., a single daily dose of 0.5% Tween-80 (1ml) on all five days. Group II, the APAP control animals were administered a single daily dose of 0.5%Tween 80 (1ml) p.o., on all the 5 days a
机译：传统的印度药用植物印度菊（Sphaeranthus indicus Linn）的花头通常用于滋养和改善肝脏状况。这项研究的目的是评估印度梧桐花头的水性（AQS）和甲醇（MES）提取物对扑热息痛（APAP）诱导的大鼠体内肝毒性的保护作用。口服MES（300mg / kg）时，肝脏标志物酶，谷氨酸-草酰乙酸转氨酶（SGOT），谷氨酸-丙酮酸转氨酶（SGPT），酸性磷酸酶（ACP）和碱性磷酸酶（ALP）胆红素和总蛋白的活性显示出显着的保肝作用与相同剂量的水提取物相比效果更好。研究治疗动物的肝脏组织病理学也证实了这一事实。至于抗氧化活性，MES表现出显着的效果（P <0.05），显示出通过降低丙二醛（MDA）含量而增加的超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物（GPX）的水平。引言保护肝脏，具有抗氧化特性的药物与诱导肝毒性的药物之间的药物失衡，促使并加速了对民间疗法中用于治疗肝脏疾病和增强肝脏功能的植物的研究。东方菊。 （菊科）在印地语中通常被称为Gorakhmundi是一种一年生蔓延草本植物，分布在印度，斯里兰卡和澳大利亚的平原和湿地。1、2据报道，整株植物都被用于印度草药的阿育吠陀系统中，癫痫和精神疾病。3据报道，它可用于治疗绒毛，肝炎4，并具有免疫抑制作用。5有关该植物的ariel部分的文献报道显示，精油中存在糖精和类杜鹃花6。从这种草药中分离出5,4'-二甲氧基-3'-异戊二烯生物素7-邻-β-半乳糖苷和一些有趣的倍半萜烯。8,9,10和新的黄酮苷11。在这项研究中，我们已经研究了印度菊苣的头状花序提取物在大鼠体内保护肝脏免受扑热息痛诱导的肝细胞损伤和氧化应激的能力。材料和方法植物材料：印度印度菊（Sereranthus indicus（SI））的干花头是从印度Meerut UP的当地药品市场采购的，并由印度新德里国家科学通信与信息资源研究所（NISCAIR）HB Singh博士鉴定。 。凭证样本存放在我们药理学实验室的植物标本室中。提取：将干粉状的头状花序机械粉化（10/44号筛），在索氏萃取仪中用甲醇将约250g的粉末用粉末萃取。在沸腾的蒸馏水中完全提取出另外的250g粉末材料。旋转真空蒸发器浓缩两种提取物，两种提取物的百分产率分别为22.5％和20.9％。将两种提取物的残余物制成分别溶于200 mg / ml的Tween-80（0.08％）的水和丙二醇（4：1）悬浮液。动物：每只体重分别为180-200g的30只无病原体的雄性白化病大鼠（四只虚弱）用于研究。它们被放置在特定的标准实验室条件下，并保持在温度控制环境（25±10C）中，相对湿度（55±5％）中，并有规律的12h亮/ 12h暗周期。所有动物均喂食标准大鼠食物，随意饮水并按照机构伦理委员会的规定接受人道关怀。保肝活性：将大鼠分为五组，每组六只。第一组，正常对照组动物，在全部五天中，每天口服一次0.5％Tween-80（1ml）的每日剂量。第II组，APAP对照动物在每天的5天内，每天口服0.5％吐温80（1ml）的单日剂量。

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