...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Internet Journal of Toxicology >Bergenin, A Nigerian Alcoholic Beverage Additive From Sacoglottis Gabonensis As An Antioxidant Protector Of Mammalian Cells Against 2,4—Dinitrophenyl Hydrazine-Induced Lipid Peroxidation
【24h】

Bergenin, A Nigerian Alcoholic Beverage Additive From Sacoglottis Gabonensis As An Antioxidant Protector Of Mammalian Cells Against 2,4—Dinitrophenyl Hydrazine-Induced Lipid Peroxidation

机译：卑尔根（Seroglottis Gabonensis）的尼日利亚酒精饮料添加剂，作为哺乳动物细胞抗2,4-二硝基苯基肼诱导的脂质过氧化的抗氧化剂

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of bergenin isolated from Sacoglottis gabonensis bark extract, on membrane lipid peroxidation and tissue ascorbic acid level was studied using rat as the experimental animal and 2,4-dinitrophenyl as the experimental oxidant.Pretreatment with bergenin significantly reduced but did not completely abolish DNPH-induced lipid peroxidation in the liver,brain and red blood cell as evidenced from the levels of the two intermediates, lipid hydroperoxide and aldehydes, measured. Bergenin also protected against DNPH-induced depletion of tissue ascorbic acid and had a sparing effect in untreated animals. The results suggest that bergenin may be responsible for the earlier observed anti-lipid peroxidizing effect of the bark extract. Introduction Sacoglottis gabonensis stem bark is commonly used as palm wine additive in some rainforest communities of Southern Nigeria. Laboratory studies have shown that the extract bergenin (Fig 1) may possess antioxidant properties (Ekong and Ejike 1974, Madusolumuo, 1993; Okechukwu 1998). Ekong and Ejike reported that bergenin isolated from the bark extract had antioxidant properties towards yeast alcohol metabolism. A similar observation was made with the crude extract by Okechukwu (1998) who demonstrated that it was a non-competitive inhibitor of yeast alcohol and aldehyde dehydrogenases. Madusolumuo (1993) studied the effect of the bark extract in mammalian system and found that pretreatment with the additive protected the rat liver from acetaminophen- induced cytotoxicity. We have recently examined the antioxidant activity of the crude extract in the mammalian system in detail using 2,4-dinitrophenyl hydrazine as the experimental peroxidant. The crude extract was found to effectively inhibit experimental lipid peroxidation in the liver, brain and red blood cells as determined by the tissue concentrations of three stable intermediates of the lipid peroxidation pathway, lipid hydroperoxide, lipid hydroperoxide carbonyl and lipid aldehyde (Maduka and Okoye 2002a). The crude extract was subsequently shown to exert a sparing effect on tissue antioxidant vitamins, ascorbic acid and vitamin E, protecting them against the 2,4-DNPH-induced depletion in the liver, brain and red blood cell, and to remove the inhibitory effect of 2,4-DNPH on the activity of the antioxidant enzyme, superoxide dismutase (Maduka and Okoye 2002b).
机译：以大鼠为实验动物，以2,4-二硝基苯基为实验氧化剂，研究了加蓬树皮提取物中的佛手素对膜脂质过氧化和组织抗坏血酸水平的影响，其中佛手素的预处理可显着减少但并未完全消除DNPH。从两种中间产物脂质氢过氧化物和醛的水平可以证明，β-羟色胺在肝，脑和红细胞中引起脂质过氧化。 Bergenin还可以防止DNPH诱导的组织抗坏血酸消耗，并且在未经治疗的动物中具有保护作用。结果表明，佛手素可能是树皮提取物早期观察到的抗脂质过氧化作用的原因。简介Sacoglottis gabonensis茎皮在尼日利亚南部的一些雨林社区中通常用作棕榈酒的添加剂。实验室研究表明，提取的佛手素（图1）可能具有抗氧化特性（Ekong和Ejike，1974; Madusolumuo，1993; Okechukwu，1998）。 Ekong和Ejike报道，从树皮提取物中分离出的佛手素对酵母酒精代谢具有抗氧化特性。 Okechukwu（1998）对粗提物进行了类似的观察，结果表明它是酵母醇和醛脱氢酶的非竞争性抑制剂。 Madusolumuo（1993）研究了树皮提取物在哺乳动物系统中的作用，发现用添加剂预处理可保护大鼠肝脏免受对乙酰氨基酚诱导的细胞毒性作用。我们最近使用2，4-二硝基苯基肼作为实验性过氧化物，详细研究了粗提物在哺乳动物系统中的抗氧化活性。已发现粗提取物可有效抑制肝脏，大脑和红细胞中的实验性脂质过氧化，这取决于脂质过氧化途径的三种稳定中间体，脂质氢过氧化物，脂质氢过氧化物羰基和脂质醛的组织浓度（Maduka和Okoye 2002a ）。粗提物随后显示出对组织抗氧化剂维生素，抗坏血酸和维生素E的保护作用，从而保护它们免受2,4-DNPH诱导的肝，脑和红细胞消耗，并消除抑制作用2,4-DNPH对抗氧化酶，超氧化物歧化酶活性的影响（Maduka和Okoye 2002b）。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号