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首页> 外文期刊>The Internet journal of veterinary medicine >Effect of heavy metal pollution of water on total plasma proteins and serum protein profiles of Common Carp fish (Cyprinus carpio)
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Effect of heavy metal pollution of water on total plasma proteins and serum protein profiles of Common Carp fish (Cyprinus carpio)

机译：水的重金属污染对鲤鱼总血浆蛋白和血清蛋白谱的影响

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Two groups of six Common Carp fish each were exposed to a combination of various compounds of eight heavy metals in water for two months. The concentration of the metals in the water was the same as found in the polluted water of Buddha Nallah, a drain of Ludhiana which flows through the heart of the city and joins the river Satluj. Levels of total plasma proteins and serum protein profiles of normal and pollutant – exposed fish were analyzed. The mean value of total plasma proteins was lower (though non – significantly) in case of pollutant – exposed fish. Serum protein profiles on SDS – PAGE showed that the proteins p63 and p25 disappear while p56 and p22 appear in the sera of fish on exposure to heavy metal polluted water. Introduction The normal functions of fish are susceptible to adverse changes in water quality. Occurrence of aquatic pollutants (such as heavy metals) has been correlated to alterations in the fish immune system and the incidence of infectious diseases. Even very low sub lethal doses of certain heavy metals can have profound effects upon the structure and / or functions of the immune system that could be almost as harmful as direct toxic doses. Ludhiana has a large number of industries for manufacturing bicycle parts, Nickel – Chrome plating, dyeing and woolen hosiery etc. The heavy metal Chromium is often found in the effluents of many industries especially Chrome plating and Chrome tanning industries which are a major source of pollution of surface and ground water. Pollution of water with heavy metals may adversely affect the immune system of fish leading to decreased production, increased susceptibility to diseases and mortality. The effect of heavy metals on immunity of common carp fish was, therefore, evaluated in this study. Materials and methods Experimental fish: The present studies were conducted on Common Carp (Cyprinus carpio) 20 cm long and weighing 250 gm available at GADVASU Fish Farm, Ludhiana. Exposure of fish to heavy metals: Fishes were divided into two groups of 10 fish each and kept in plastic tanks of size 160 x 110 x 110 cm. Aerators and ample daylight were provided in the tanks and the water temperature was maintained at 30 0 C. The fishes were acclimatized under lab conditions for two weeks and then exposed to 8 heavy metals in the concentration (mg/L) as found in the polluted water of Buddha Nallah (Iron – 35.86; Zinc – 2.75; Manganese – 0.166; Nickel – 0.090; Chromium – 0.065; Copper – 0.045; Lead – 0.044; Cadmim – 0.025, respectively). The fish were fed daily with normal fish feed @ 1% of their body weight. Estimation of plasma total protein levels: Estimation of total protein levels in the plasma of normal and pollutant – exposed fish was done by Lowry's method. Serum protein profiles: Serum protein profiles were studied by SDS Polyacrylamide gel electrophoresis (SDS PAGE) as per the standard method using 5% gel for stacking and 12.5% gel for resolving of proteins. Results Levels of total proteins in plasma: The total protein concentrations in the plasma of normal and pollutant – exposed fish were determined. The mean ± SE value of concentration of proteins (μg/ml) in the plasma was 396.17 ± 26.50 in normal fish and 323.00 ± 18.41 in pollutant – exposed fish. Although the mean value was lower in case of pollutant – exposed fish, it did not differ significantly from that of normal fish (Table 1).
机译：将两组六只普通鲤鱼分别暴露于水中的八种重金属的各种化合物的混合作用两个月。水中的金属浓度与佛陀纳拉（Ludhiana的排水渠）中被污染的水相同，佛陀纳拉排水渠流经城市中心并与萨特鲁吉河汇合。分析了正常和受污染鱼类的总血浆蛋白水平和血清蛋白谱。在暴露于污染物的鱼的情况下，血浆总蛋白的平均值较低（尽管不是很明显）。在SDS – PAGE上的血清蛋白谱显示，暴露于重金属污染的水中，鱼血清中的蛋白p63和p25消失，而p56和p22出现。引言鱼的正常功能容易受到水质不利变化的影响。水生污染物（例如重金属）的发生与鱼类免疫系统的改变和传染病的发生有关。甚至某些重金属的极低致死剂量也可能对免疫系统的结构和/或功能产生深远的影响，其危害几乎与直接毒性剂量相同。卢迪亚纳（Ludhiana）有许多制造自行车零件的行业，包括镍–铬电镀，染色和羊毛袜业等。许多行业的废水中经常发现重金属铬，特别是铬电镀和铬鞣革行业是造成污染的主要来源。地表水和地下水。重金属污染水可能会对鱼类的免疫系统产生不利影响，从而导致产量下降，对疾病和死亡率的敏感性增加。因此，本研究评估了重金属对鲤鱼免疫力的影响。材料和方法实验鱼：本研究是在20厘米长，重250克的鲤鱼（鲤鱼）上进行的，该鱼可从卢迪亚纳的GADVASU鱼场获得。鱼暴露于重金属中：将鱼分成两组，每组10条鱼，并保存在160 x 110 x 110厘米大小的塑料罐中。池子中装有曝气器和充足的日光，水温保持在30 0C。在实验室条件下使鱼类适应环境两周，然后将其暴露于8种重金属（浓度为mg / L），如污染佛陀纳拉水（分别为铁– 35.86;锌– 2.75;锰– 0.166;镍– 0.090;铬– 0.065;铜– 0.045;铅– 0.044;镉-0.025）。每天用正常鱼饲料（体重的1％）喂鱼。血浆总蛋白水平的估算：正常鱼和污染物暴露鱼类的血浆中总蛋白水平的估算是通过Lowry方法进行的。血清蛋白谱：按照标准方法通过SDS聚丙烯酰胺凝胶电泳（SDS PAGE）研究血清蛋白谱，使用5％凝胶进行堆叠，使用12.5％凝胶进行蛋白质分离。结果血浆中总蛋白水平：测定正常和污染物接触鱼类的血浆中总蛋白浓度。正常鱼的血浆蛋白浓度（μg/ ml）的平均值±SE值在正常鱼中为396.17±26.50，在污染物暴露的鱼中为323.00±18.41。尽管暴露于污染物的鱼类的平均值较低，但与正常鱼类的平均值没有显着差异（表1）。

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