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首页> 外文期刊>The Internet Journal of Toxicology >In vitro and in vivo evaluation of toxic effect of benzene on lymphocytes and hepatocytes.
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In vitro and in vivo evaluation of toxic effect of benzene on lymphocytes and hepatocytes.

机译：体外和体内苯对淋巴细胞和肝细胞毒性作用的评估。

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Objective: Explore the hepato and hematotoxicity of benzene through in vitro and in vivo evaluation.Methods: Spleenic lymphocytes and hepatocytes were used for the in vitro study. Liver enzymology and lymphocytes were evaluated in 154 petrol filling workers with <10 years and >10 years of exposure and 33 referents.Results: Lymphocytopenia was most marked in study group than in controls. Absolute lymphocyte count were reduced to 1.67±0.3 x 103/mL and 1.49 ±0.4 x 103/mL in workers with <10 years and >10years of exposure than the control (1.9 ±0.3 x 103/mL). This was supported by our in vitro result showing a negative correlation between reduction in viability of lymphocytes and increasing concentration of benzene. The mean value of ALP and ALT in study group was significantly lowered than in control group. The total protein level increased within the normal range in study group than in controls whereas albumin level decreased in study group in contrast with control group. Mean ± SD values of AST and total bilirubin in study group were not elevated from the corresponding value in control group.Conclusion: These in vitro and in vivo results show that human lymphocytes and hepatocytes are sensitive targets of benzene. Introduction Benzene is a ubiquitous industrial solvent and widely distributed environmental contaminant that has been linked to adverse health effect in humans and animals [1-3]. Benzene ranks as the 17th chemical in terms of total annual production in 1994 [4] and therefore represents a significant occupational hazard. Hematotoxicity is the most noted and characteristic systemic effect resulting from intermediate and chronic benzene exposure leading to aplastic anemia, leucopenia and thrombocytopenia [5,6]. Other health effects of benzene include immunological changes which appear largely related to decrease in circulating leukocytes and the ability of lymphoid tissue to produce mature lymphocytes necessary to form antibodies [7,8]. Chronic exposure results in consistent structural and numerical chromosomal aberrations in lymphocytes and bone marrow cells. Benzene metabolites covalently bind to cellular macromolecules (including DNA, RNA and proteins) thereby leading to dysfunction in the bone marrow and other tissues. Along with benzene, one or more reactive benzene metabolites are involved in the health effects. Majority of benzene metabolism occurs in liver by cytochrome P450 and a small amount is metabolized in the bone marrow [9-11], the site of characteristic benzene toxicity (Figure 1).
机译：目的：通过体外和体内评价探讨苯的肝毒性和血液毒性。方法：采用脾脏淋巴细胞和肝细胞进行体外研究。在154名暴露<10年和> 10年的汽油加油工人和33名受试者中评估了肝脏酶学和淋巴细胞。结果：研究组的淋巴细胞减少症最明显。与对照组相比，暴露<10年和> 10年的工人的绝对淋巴细胞计数分别降低至1.67±0.3 x 103 / mL和1.49±0.4 x 103 / mL（1.9±0.3 x 103 / mL）。我们的体外结果支持了这一点，该结果表明淋巴细胞活力的降低与苯浓度的增加之间呈负相关。研究组的ALP和ALT均值明显低于对照组。与对照组相比，研究组的总蛋白水平在正常范围内增加，而对照组的白蛋白水平下降。研究组的AST和总胆红素的平均值±SD值没有比对照组的相应值高。结论：这些体内和体外结果表明，人淋巴细胞和肝细胞是苯的敏感靶标。引言苯是一种普遍存在的工业溶剂，是分布广泛的环境污染物，与人类和动物的不良健康影响有关[1-3]。苯在1994年的年总产量中排名第17位[4]，因此代表了重大的职业危害。血液毒性是中度和慢性苯暴露引起的再生障碍性贫血，白细胞减少症和血小板减少症引起的最明显和最典型的全身性作用[5,6]。苯对健康的其他影响包括免疫学变化，这些变化在很大程度上与循环白细胞的减少以及淋巴组织产生形成抗体所需的成熟淋巴细胞的能力有关[7,8]。长期暴露会导致淋巴细胞和骨髓细胞中一致的结构和数值染色体畸变。苯代谢物与细胞大分子（包括DNA，RNA和蛋白质）共价结合，从而导致骨髓和其他组织功能障碍。与苯一起，一种或多种反应性苯代谢物也参与健康影响。苯代谢的大部分发生在肝脏中的细胞色素P450，少量代谢在骨髓中[9-11]，苯是特征性苯中毒的部位（图1）。

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