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首页> 外文期刊>The Journal of Veterinary Medical Science >Expressions of Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor in Tumors Induced by Two Different Cloned Cell Lines Established from Transplantable Rat Malignant Fibrous Histiocytoma
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Expressions of Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor in Tumors Induced by Two Different Cloned Cell Lines Established from Transplantable Rat Malignant Fibrous Histiocytoma

机译：移植性大鼠恶性纤维组织细胞瘤建立的两种不同克隆细胞系诱导的肿瘤中血管内皮生长因子和碱性成纤维细胞生长因子的表达

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References(38) Cited-By(2) In order to establish base-line data on angiogenic factors in development of mesenchymal tumors, expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in implanted MT-8 and MT-9 tumors, both derived from a transplantable malignant fibrous histiocytoma (MFH) in the F344 rat, were investigated by immunohistochemistry and Western blotting method. MT-8 and MT-9 tumors were developed in syngeneic rats by implant of a tumor tissue fragment. MT-8 tumors were examined on post-implantation (PI) days 3, 6, 9 and 17, and MT-9 tumors were on PI days 9, 14, 17 and 23. The growth of MT-8 tumors was faster than that of MT-9 tumors. Histologically, MT-8 tumors were features of undifferentiated sarcomas, whereas MT-9 tumors exhibited a typical storiform growth pattern of MFH. Immunohistochemically, all cells constituting MT-8 and MT-9 tumors reacted with antibodies to VEGF and bFGF, indicating production of these factors by mesenchymal neoplastic cells. However, there were no marked differences in these immunoreactions between tumors examined. Thus, the bands obtained in the Western blotting methods were densitometrically scanned. The expression levels of VEGF and bFGF gradually increased PI day 3 to 9 in MT-8 tumors and PI day 9 to 17 in MT-9 tumors. On last examination day, the levels of bFGF in both tumors and of VEGF in MT-9 tumors decreased, but the VEGF expression level in MT-8 tumors was still increased. These findings indicated that VEGF and bFGF may contribute cooperatively to angiogenesis in an early growth of mesenchymal tumor development.
机译：参考文献（38）Cited-By（2）为了建立间充质肿瘤发展过程中血管生成因子的基线数据，在植入的MT-8和VEGF中表达血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）。通过免疫组化和Western印迹方法研究了均来自F344大鼠中可移植的恶性纤维组织细胞瘤（MFH）的MT-9肿瘤。 MT-8和MT-9肿瘤是通过植入肿瘤组织片段在同系大鼠中发展而来的。在植入后（PI）第3、6、9和17天检查了MT-8肿瘤，在PI第9、14、17和23天检查了MT-9肿瘤。MT-8肿瘤的生长速度快于MT-9肿瘤。从组织学上讲，MT-8肿瘤是未分化肉瘤的特征，而MT-9肿瘤则表现出MFH的典型星形生长模式。在免疫组织化学上，构成MT-8和MT-9肿瘤的所有细胞均与VEGF和bFGF抗体发生反应，表明这些因子是由间充质瘤细胞产生的。然而，在所检查的肿瘤之间，这些免疫反应没有显着差异。因此，对通过蛋白质印迹法获得的条带进行光密度扫描。 VEGF和bFGF的表达水平在MT-8肿瘤的PI第3至9天和MT-9肿瘤的PI第9至17天逐渐增加。在最后检查日，两种肿瘤中的bFGF水平和MT-9肿瘤中的VEGF水平均降低，但MT-8肿瘤中的VEGF表达水平仍升高。这些发现表明，VEGF和bFGF可能在间充质肿瘤发展的早期生长中共同促进血管生成。

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