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首页> 外文期刊>The Journal of Veterinary Medical Science >A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells
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A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells

机译：犬骨髓间充质干细胞与脂肪间充质干细胞神经球分化潜能的比较

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References(41) Cited-By(9) Stem cell transplantation is one of the most promising yet enigmatic treatments for spinal cord injury (SCI), a common problem in dogs. As pre-differentiated mesenchymal stem cells (MSCs) can be expanded and differentiated into neurospheres in vitro, before being transplanted back, they may prove to be more beneficial for treating SCI. Therefore, we compared the endogenous differentiation potential, including the neuronal cell differentiation, of neurospheres from canine bone marrow MSCs (cBMMSCs) with that of the adipose tissue-derived MSCs (cADMSCs). Nestin-positive neurospheres were generated from MSCs derived from the bone marrow and adipose tissue. Neuronal cells were differentiated from the neurospheres derived from both these tissues. Gene expression analysis revealed that Nestin, βIII-tubulin, NCAM, OCT4 and SOX2 were expressed in MSCs and the corresponding neurospheres. Notably, cBMMSC-derived neuronal cells expressed higher levels of βIII-tubulin. The mRNA expressions of NANOG, Nestin, OCT4 and SOX2 were upregulated in neurospheres derived from both. Immunofluorescence analysis detected the expression of neuronal markers, namely, βIII-tubulin, GFAP, S100, NF200 and MAP2, in differentiated neuron-like cells. Our findings highlight that both cBMMSCs and cADMSCs could be differentiated into neurospheres and neuron-like cells, and therefore, these cells are suitable candidates for cell transplantation. Further, cADMSCs form a more suitable cell source, as larger number of cells could be harvested from cADMSC-derived neurospheres. Future studies employing in vivo transplantation models to investigate the effectiveness of MSCs for treating SCI are warranted.
机译：参考文献（41）Cited-By（9）干细胞移植是脊髓损伤（SCI）（一种狗的常见问题）中最有前途但仍令人迷惑的治疗方法之一。由于预分化的间充质干细胞（MSC）可以在体外扩增并分化为神经球，然后再移植回去，因此它们可能被证明对治疗SCI更有益。因此，我们比较了来自犬骨髓MSC（cBMMSC）和脂肪组织来源MSC（cADMSC）的神经球的内源性分化潜能，包括神经元细胞的分化。 Nestin阳性神经球是从骨髓和脂肪组织的MSC产生的。将神经元细胞与源自这两种组织的神经球区分开。基因表达分析表明，巢蛋白，βIII-微管蛋白，NCAM，OCT4和SOX2在MSC和相应的神经球中表达。值得注意的是，源自cBMMSC的神经元细胞表达更高水平的βIII-微管蛋白。在两种来源的神经球中，NANOG，Nestin，OCT4和SOX2的mRNA表达均上调。免疫荧光分析检测到分化的神经元样细胞中神经元标志物，即βIII-微管蛋白，GFAP，S100，NF200和MAP2的表达。我们的发现表明，cBMMSC和cADMSC都可以分化为神经球和神经元样细胞，因此，这些细胞适合用于细胞移植。此外，由于可从cADMSC衍生的神经球中收获大量细胞，因此cADMSC形成了更合适的细胞来源。将来有必要采用体内移植模型来研究MSC治疗SCI的有效性。

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