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首页> 外文期刊>The Korean Journal of Parasitology >Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography
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Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography

机译：中国广西肝和肠小肠人类感染：DNA测序，超声检查和免疫亲和层析分析

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The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi, China in May 2010. A total of 718 people were examined for helminth eggs by the Kato-Katz thick smear technique, ultrasonography, immunoaffinity chromatography, and DNA sequencing. The overall egg positive rate was found to be 59.6% (28.0-70.6%) that included mixed infections with liver and intestinal flukes. Cases showing higher than 20,000 eggs per gram of feces (EPG) were detected between 1.3% and 16.2%. Ultrasonographic findings exhibited overall 28.2% (72 of 255 cases) dilatation rate of the intrahepatic bile duct. Clonorchis sinensis infection was detected serologically in 88.3% (38 of 43 cases) among C. sinensis egg positive subjects by the immunoaffinity chromatography using a specific antigen for C. sinensis. For differential diagnosis of the liver and intestinal flukes, more precise PCR and nucleotide sequencing for copro-DNA were performed for 46 egg positive cases. Mixed infections with C. sinensis and Metagonimus yokogawai were detected in 8 of 46 egg positive cases, whereas 29 specimens were positive for Haplorchis taichui. Ultrasonographic findings and immunoaffinity chromatography results showed usefulness, even in a limited way, in figuring out of the liver fluke endemicity.
机译：肝和肠吸虫感染的流行率是通过调查2010年5月在中国广西广为人知的肝吸虫流行的横轩，扶s和上林村的居民来确定的。卫生部检查了718人的蠕虫卵。 Kato-Katz浓涂技术，超声检查，免疫亲和层析和DNA测序。发现总的鸡蛋阳性率为59.6％（28.0-70.6％），其中包括肝和肠吸虫的混合感染。检出的鸡蛋中每克粪便（EPG）高于20,000个的病例在1.3％至16.2％之间。超声检查结果显示肝内胆管总体扩张率为28.2％（255例中的72例）。使用中华绒螯蟹特异性抗原的免疫亲和层析技术在中华绒螯蟹卵阳性患者中，血清学检测到中华支睾吸虫感染率为88.3％（43例中的38例）。为了鉴别肝吸虫和肠道吸虫，对46例鸡蛋阳性病例进行了更精确的PCR和copro-DNA核苷酸测序。在46例鸡蛋阳性病例中，有8例检出了C. sinensis和yokogawai混合感染，而29例标本为太白杆菌。超声检查结果和免疫亲和层析结果表明，即使在有限的方式下，也可用于排除肝吸虫流行。

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