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首页> 外文期刊>The Korean Journal of Parasitology >Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene
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Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene

机译：利用GRA20基因序列分析弓形虫不同宿主和地理区域的遗传多样性

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摘要

Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.
机译：弓形虫是蚜虫门的真核寄生虫，它会感染所有温血动物，包括人。在本研究中，我们检查了从全球不同宿主和地理区域收集的弓形虫分离株中致密颗粒20（GRA20）基因的序列变异。使用PCR从16个刚地弓形虫菌株中扩增出完整的GRA20基因，对其序列进行了分析，并通过最大简约法（MP）和最大似然法（ML）分析了系统发育重建。结果表明，在本研究中使用的所有分离株中，GRA20基因的完整序列的长度为1586 bp，在所有菌株中，核苷酸的序列变异为0-7.9％。然而，除去III型菌株（CTG，VEG），序列变异变得非常低，仅为0-0.7％。这些结果表明，III型的GRA20序列有更大的差异。使用MP和ML方法进行的GRA20序列的系统发生分析可以将2种主要的克隆谱系类型（I型和III型）区分为各自的簇，这表明GRA20基因可能代表了弓形虫种内系统发育分析的新型遗传标记。

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