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首页> 外文期刊>The Journal of Veterinary Medical Science >Detection of Salmonella Gallinarum and S. Typhimurium DNA in Experimentally Infected Chicks by Polymerase Chain Reaction
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Detection of Salmonella Gallinarum and S. Typhimurium DNA in Experimentally Infected Chicks by Polymerase Chain Reaction

机译：聚合酶链反应检测实验感染小鸡沙门氏菌和鼠伤寒沙门氏菌DNA

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References(22) Cited-By(4) DNA detection with polymerase chain reaction (PCR) as a mean of identifying Salmonella infection in chickens was compared with the conventional culture procedure. DNA was extracted from organs of experimentally infected chicks with either S. Gallinarum or S. Typhimurium. The pair of primers used were those directed at the InvA gene. Bacteria isolation was done by inoculating the pre-enrichment media with samples. As was expected a 284 bp fragment DNA was amplified from extracted DNA of infected organs by PCR. The results of our studies indicate that the PCR method is more sensitive than the conventional culture procedure since we were able to detect both S. Gallinarum and S. Typhimurium DNA not only in samples positive for bacteria isolation but also in negative samples. It was possible to detect Salmonella DNA in 15 out of 20 organ samples from chicks infected with S. Gallinarum 21 hr after infection, but, only five were positive for bacteria isolation. Salmonella DNA was detected throughout the entire test period. The results of this study confirm that PCR is a useful tool for the detection of Salmonella infection in poultry.
机译：参考文献（22）将利用聚合酶链反应（PCR）进行DNA引物检测（By（4））作为鉴定鸡沙门氏菌感染的手段与常规培养方法进行了比较。从实验感染的鸡鸡的S. Gallinarum或鼠伤寒沙门氏菌的器官中提取DNA。使用的引物对是针对InvA基因的那些。细菌的分离是通过在富集前的培养基中接种样品来完成的。如预期的那样，通过PCR从感染器官的提取DNA中扩增了284bp的片段DNA。我们的研究结果表明，PCR方法比常规培养过程更为灵敏，因为我们不仅能够在细菌分离阳性的样本中而且在阴性样本中都能检测到鸡沙门氏菌和鼠伤寒沙门氏菌DNA。感染21小时后，在感染鸡痢疾链球菌的雏鸡的20个器官样本中，有15个可以检测到沙门氏菌DNA，但只有5个细菌分离阳性。在整个测试期间都检测到沙门氏菌DNA。这项研究的结果证实，PCR是检测家禽沙门氏菌感染的有用工具。

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