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首页> 外文期刊>The Journal of Veterinary Medical Science >Epidemiological Survey of Theileria orientalis Infection in Grazing Cattle in the Eastern Part of Hokkaido, Japan
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Epidemiological Survey of Theileria orientalis Infection in Grazing Cattle in the Eastern Part of Hokkaido, Japan

机译：日本北海道东部放牧牛的东方泰勒虫感染的流行病学调查

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References(28) Cited-By(48) Theileria orientalis is one of the benign species of Theileria that is widely distributed in Japan and is sometimes responsible for serious economic losses in the livestock industry. In the present study, we surveyed the current status of T. orientalis infection in grazing cattle in the eastern areas of Hokkaido (Taiki, Otofuke, Shintoku, and Shin-Hidaka districts) using molecular methods, as well as traditional methods, of diagnosis. The genes encoding the major piroplasm surface protein (MPSP) and p23 of T. orientalis were identified using highly detectable polymerase chain reaction (PCR). Results of the MPSP-PCR assay indicated that grazing cattle in these districts, after about 1.5 months pasturage, showed high rates of infection, ranging from 10.0-64.8%. Although the main MPSP and p23 genotypes detected were the Ikeda- or Chitose-types, an MPSP gene closely relating to that found in Okinawa prefecture, and a p23 gene closely relating to the Australian (Warwick) Buffeli-type gene, were found in the cattle in Shintoku and Shin-Hidaka districts. The present survey indicated that there were at least five types of T. orientalis classified by their MPSP genes in Hokkaido, Japan, and that T. orientalis infection rates are still high in this region.
机译：参考文献（28）被引用的（48）东方泰勒虫是在日本广泛分布的泰勒虫的良种之一，有时对畜牧业造成严重的经济损失。在本研究中，我们使用分子方法以及传统方法对北海道东部地区（Taiki，Otofuke，Shintoku和Shin-Hidaka地区）的放牧牛的东方锥虫感染现状进行了调查。使用高度可检测的聚合酶链反应（PCR）鉴定了编码东方侧柏主要蛋白表面蛋白（MPSP）和p23的基因。 MPSP-PCR分析的结果表明，在这些地区放牧约1.5个月后，这些地区的放牧牛表现出较高的感染率，范围为10.0-64.8％。尽管检测到的主要MPSP和p23基因型是池田型或千岁型，但在冲绳县发现了与该基因密切相关的MPSP基因和与澳大利亚（沃里克）布菲利型基因密切相关的p23基因。新得区和新日高区的牲畜。目前的调查表明，在日本北海道，至少有五种按其MPSP基因分类的东方侧柏，而该区域的东方侧柏感染率仍然很高。

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