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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Toll-like Receptor 2 is Dispensable for an Immediate-early Microglial Reaction to Two-photon Laser-induced Cortical Injury In vivo
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Toll-like Receptor 2 is Dispensable for an Immediate-early Microglial Reaction to Two-photon Laser-induced Cortical Injury In vivo

机译：Toll样受体2是必需的立即早期的小胶质细胞反应到体内的双光子激光诱导皮层损伤。

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Microglia, the resident macrophages in the central nervous system, can rapidly respond to pathological insults. Toll-like receptor 2 (TLR2) is a pattern recognition receptor that plays a fundamental role in pathogen recognition and activation of innate immunity. Although many previous studies have suggested that TLR2 contributes to microglial activation and subsequent pathogenesis following brain tissue injury, it is still unclear whether TLR2 has a role in microglia dynamics in the resting state or in immediate-early reaction to the injury in vivo . By using in vivo two-photon microscopy imaging and Cx3cr1 GFP/+ mouse line, we first monitored the motility of microglial processes (i.e. the rate of extension and retraction) in the somatosensory cortex of living TLR2-KO and WT mice; Microglial processes in TLR2-KO mice show the similar motility to that of WT mice. We further found that microglia rapidly extend their processes to the site of local tissue injury induced by a two-photon laser ablation and that such microglial response to the brain injury was similar between WT and TLR2-KO mice. These results indicate that there are no differences in the behavior of microglial processes between TLR2-KO mice and WT mice when microglia is in the resting state or encounters local injury. Thus, TLR2 might not be essential for immediate-early microglial response to brain tissue injury in vivo .
机译：小胶质细胞是中枢神经系统中的常驻巨噬细胞，可以对病理损伤迅速做出反应。 Toll样受体2（TLR2）是一种模式识别受体，在病原体识别和先天免疫激活中起着基本作用。尽管许多先前的研究表明TLR2有助于脑组织损伤后小胶质细胞的活化和随后的发病机制，但仍不清楚TLR2是否在静止状态的小胶质细胞动力学或体内对损伤的早期反应中起作用。通过使用体内双光子显微镜成像和Cx3cr1 GFP / + 小鼠系，我们首先监测了活的TLR2-KO体感皮层中小胶质细胞过程的运动性（即伸展和收缩的速率）和野生型小鼠; TLR2-KO小鼠的小胶质细胞过程显示出与WT小鼠相似的运动能力。我们进一步发现，小胶质细胞迅速将其过程扩展到由双光子激光消融诱导的局部组织损伤部位，并且这种小胶质细胞对脑损伤的反应在WT和TLR2-KO小鼠之间相似。这些结果表明，当小胶质细胞处于静止状态或受到局部损伤时，在TLR2-KO小鼠和WT小鼠之间，小胶质细胞过程的行为没有差异。因此，TLR2对于体内早期对脑组织损伤的小胶质细胞反应可能不是必需的。

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