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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Decreased Expression of Na+/K+-ATPase, NHE3, NBC1, AQP1 and OAT in Gentamicin-induced Nephropathy
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Decreased Expression of Na+/K+-ATPase, NHE3, NBC1, AQP1 and OAT in Gentamicin-induced Nephropathy

机译：庆大霉素诱发的肾病中Na + / K + -ATPase，NHE3，NBC1，AQP1和OAT的表达降低

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The present study was aimed to determine whether there is an altered regulation of tubular transporters in gentamicin-induced nephropathy. Sprague-Dawley male rats (200~250 g) were subcutaneously injected with gentamicin (100 mg/kg per day) for 7 days, and the expression of tubular transporters was determined by immunoblotting and immunohistochemistry. The mRNA and protein expression of OAT was also determined. Gentamicin-treated rats exhibited significantly decreased creatinine clearance along with increased plasma creatinine levels. Accordingly, the fractional excretion of sodium increased. Urine volume was increased, while urine osmolality and free water reabsorption were decreased. Immunoblotting and immunohistochemistry revealed decreased expression of Na+/K+-ATPase, NHE3, NBC1, and AQP1 in the kidney of gentamicin-treated rats. The expression of OAT1 and OAT3 was also decreased. Gentamicin-induced nephropathy may at least in part be causally related with a decreased expression of Na+/K+-ATPase, NHE3, NBC1, AQP1 and OAT.
机译：本研究旨在确定庆大霉素诱导的肾病中肾小管转运蛋白的调节是否发生改变。 Sprague-Dawley雄性大鼠（200〜250 g）皮下注射庆大霉素（每天100 mg / kg），持续7天，并通过免疫印迹和免疫组化方法检测肾小管转运蛋白的表达。还确定了OAT的mRNA和蛋白质表达。庆大霉素治疗的大鼠显示肌酐清除率明显降低，血浆肌酐水平升高。因此，钠的排泄分数增加。尿量增加，而尿渗透压和自由水重吸收减少。免疫印迹和免疫组织化学分析显示，庆大霉素治疗的大鼠肾脏中Na + / K + -ATPase，NHE3，NBC1和AQP1的表达降低。 OAT1和OAT3的表达也降低了。庆大霉素诱发的肾病可能至少部分与Na + / K + -ATPase，NHE3，NBC1，AQP1和OAT的表达降低有因果关系。

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