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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Acidic pH-activated Cl- Current and Intracellular Ca2+ Response in Human Keratinocytes
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Acidic pH-activated Cl- Current and Intracellular Ca2+ Response in Human Keratinocytes

机译：酸性pH激活的Cl-电流和人角质形成细胞中的细胞内Ca 2+响应

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The layers of keratinocytes form an acid mantle on the surface of the skin. Herein, we investigated the effects of acidic pH on the membrane current and [Ca2+]c of human primary keratinocytes from foreskins and human keratinocyte cell line (HaCaT). Acidic extracellular pH (pHe≤ 5.5) activated outwardly rectifying Cl- current (ICl,pH) with slow kinetics of voltage-dependent activation. ICl,pH was potently inhibited by an anion channel blocker 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS, 73.5% inhibition at 1 μM). ICl,pH became more sensitive to pHe by raising temperature from 24℃ to 37℃. HaCaT cells also expressed Ca2+-activated Cl- current (ICl,Ca), and the amplitude of ICl,Ca was increased by relatively weak acidic pHe (7.0 and 6.8). Interestingly, the acidic pHe (5.0) also induced a sharp increase in the intracellular [Ca2+] (Δ[Ca2+]acid) of HaCaT cells. The Δ[Ca2+]acid was independent of extracellular Ca2+, and was abolished by the pretreatment with PLC inhibitor, U73122. In primary human keratinocytes, 5 out of 28 tested cells showed Δ[Ca2+]acid. In summary, we found ICl,pH and Δ[Ca2+]acid in human keratinocytes, and these ionic signals might have implication in pathophysiological responses and differentiation of epidermal keratinocytes.
机译：角质形成细胞层在皮肤表面形成酸膜。本文中，我们研究了酸性pH对包皮和人角质形成细胞系（HaCaT）的人原代角质形成细胞膜电流和[Ca 2 + ] c 的影响。酸性细胞外pH（pH e ≤5.5）被激活，向外整流Cl -电流（I Cl，pH ），具有缓慢的电压依赖性激活动力学。 I Cl，pH 被阴离子通道阻滞剂4,4'-二异硫氰基苯乙烯-2,2'-二磺酸强力抑制（DIDS，在1μM时抑制73.5％）。通过将温度从24℃升高到37℃，I Cl，pH 对pH e 变得更加敏感。 HaCaT细胞还表达Ca 2 + 激活的Cl -电流（I Cl，Ca ）和I Cl的幅度，相对弱的酸性pH e （7.0和6.8）增加了Ca 。有趣的是，酸性pH e （5.0）也会引起细胞内[Ca 2 + ]（Δ[Ca 2 + ]]急剧增加HaCaT细胞的 acid ）。 Δ[Ca 2 + ] 酸独立于细胞外Ca 2 + ，并通过PLC抑制剂U73122的预处理而被废除。在原代人角质形成细胞中，测试的28个细胞中有5个显示了Δ[Ca 2 + ] 酸。总之，我们在人角质形成细胞中发现了I Cl，pH 和Δ[Ca 2 + ] 酸，这些离子信号可能与表皮角质形成细胞的病理生理反应和分化。

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