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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Expression of Ca2+-activated K+ Channels and Their Role in Proliferation of Rat Cardiac Fibroblasts
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Expression of Ca2+-activated K+ Channels and Their Role in Proliferation of Rat Cardiac Fibroblasts

机译：Ca2 +激活的K +通道的表达及其在大鼠心脏成纤维细胞增殖中的作用

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Cardiac fibroblasts constitute one of the largest cell populations in the heart, and contribute to structural, biochemical, mechanical and electrical properties of the myocardium. Nonetheless, their cardiac functions, especially electrophysiological properties, have often been disregarded in studies. Ca2+-activated K+ (KCa) channels can control Ca2+ influx as well as a number of Ca2+-dependent physiological processes. We, therefore, attempted to identify and characterize KCa channels in rat Cardiac fibroblasts. First, we showed that the cells cultured from the rat ventricle were cardiac fibroblasts by immunostaining for discoidin domain receptor 2 (DDR-2), a specific fibroblast marker. Secondly, we detected the expression of various KCa channels by reverse transcription polymerase chain reaction (RT-PCR), and found all three family members of KCa channels, including large conductance KCa (BK-α1- and -β1~4 subunits), intermediate conductance KCa (IK), and small conductance KCa (SK1~4 subunits) channels. Thirdly, we recorded BK, IK, and SK channels by whole cell mode patch clamp technique using their specific blockers. Finally, we performed cell proliferation assay to evaluate the effects of the channels on cell proliferation, and found that the inhibition of IK channel increased the cell proliferation. These results showed the existence of BK, IK, and SK channels in rat ventricular fibroblasts and involvement of IK channel in cell proliferation.
机译：心脏成纤维细胞构成心脏中最大的细胞群之一，并有助于心肌的结构，生化，机械和电学性质。尽管如此，他们的心脏功能，尤其是电生理特性，在研究中经常被忽略。 Ca 2 + 激活的K + （K Ca ）通道可以控制Ca 2 + 的流入以及Ca 2 + 依赖的生理过程的数目因此，我们试图鉴定和表征大鼠心脏成纤维细胞中的K Ca 通道。首先，我们通过对盘状蛋白结构域受体2（DDR-2）（一种特定的成纤维细胞标志物）进行免疫染色，显示了从大鼠心室培养的细胞是心脏成纤维细胞。其次，我们通过逆转录聚合酶链反应（RT-PCR）检测了各种K Ca 通道的表达，发现了K Ca 通道的所有三个家族成员，包括大的电导K Ca （BK-α1-和-β1〜4亚基），中间电导K Ca （IK）和小电导K Ca （SK1〜4个亚单位）通道。第三，我们使用其特定的阻滞剂通过全细胞模式膜片钳技术记录了BK，IK和SK通道。最后，我们进行了细胞增殖测定，以评估通道对细胞增殖的影响，并发现抑制IK通道可增加细胞增殖。这些结果表明大鼠心室成纤维细胞中存在BK，IK和SK通道，并且IK通道参与细胞增殖。

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