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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Forskolin Enhances Synaptic Transmission in Rat Dorsal Striatum through NMDA Receptors and PKA in Different Phases
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Forskolin Enhances Synaptic Transmission in Rat Dorsal Striatum through NMDA Receptors and PKA in Different Phases

机译：佛司可林增强NMDA受体和PKA在不同阶段通过大鼠背纹状体的突触传递。

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The effect of forskolin on corticostriatal synaptic transmission was examined by recording excitatory postsynaptic currents (EPSCs) in rat brain slices using the whole-cell voltage-clamp technique. Forskolin produced a dose-dependent increase of corticostriatal EPSCs (1, 3, 10, and 30 μM) immediately after its treatment, and the increase at 10 and 30 μM was maintained even after its washout. When the brain slices were pre-treated with (DL)-2-amino-5-phosphonovaleric acid (AP-V, 100 μM), an NMDA receptor antagonist, the acute effect of forskolin (10 μM) was blocked. However, after washout of forskolin, an increase of corticostriatal EPSCs was still observed even in the presence of AP-V. When KT 5720 (5 μM), a protein kinase A (PKA) inhibitor, was applied through the patch pipette, forskolin (10 μM) increased corticostriatal EPSCs, but this increase was not maintained. When forskolin was applied together with AP-V and KT 5720, both the increase and maintenance of the corticostriatal EPSCs were blocked. These results suggest that forskolin activates both NMDA receptors and PKA, however, in a different manner.
机译：通过使用全细胞电压钳技术记录大鼠脑切片中的兴奋性突触后电流（EPSC），来检测福司可林对皮质口突触传递的影响。佛司可林在治疗后立即产生剂量依赖性皮质皮质激素EPSC（1、3、10和30μM）的增加，即使冲洗后仍保持在10和30μM的增加。当脑片用NMDA受体拮抗剂（DL）-2-氨基-5-膦酰戊酸（AP-V，100μM）预处理时，福司可林（10μM）的急性作用被阻断。但是，洗去福司高林后，即使在存在AP-V的情况下，皮层皮质EPSCs仍能增加。当通过贴片移液器应用蛋白激酶A（PKA）抑制剂KT 5720（5μM）时，福司可林（10μM）增加了皮层皮质EPSC，但这种增加没有得到维持。当福斯高林与AP-V和KT 5720一起使用时，皮质上皮EPSC的增加和维持都受到阻碍。这些结果表明毛喉素激活NMDA受体和PKA，但是，以不同的方式。

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