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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Voltage-dependent Ca2+ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach
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Voltage-dependent Ca2+ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach

机译：在刚分离的豚鼠胃Cajal（ICC）间质细胞中鉴定出电压依赖性Ca2 +电流

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摘要

The properties of voltage dependent Ca2+ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c- Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min-1, respectively, in the presence of 1 μM nifedipine. Single ICC-MY isolated by enzyme treatment also showed c- Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K+ -rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs+-rich pipette solution at V h=-80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1 μM) blocked VDIC. Therefore, we successfully isolated c- Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca2+ currents (VDCCL).
机译：研究了豚鼠胃窦肌层（ICC-MY）中分布的Cajal间质细胞（ICC）的电压依赖性Ca 2 + 电流（VDCC）。在组织中，在1μM硝苯地平存在下，ICC-MY表现出c-Kit阳性反应，并产生振幅和频率分别为约62 mV和2倍min -1 的驱动电位。通过酶处理分离的单个ICC-MY也显示出c-Kit免疫组化反应性。这些细胞还可以通过在富含K + 的移液器中产生的自发内向电流来鉴定。电压钳实验显示了在不规则频率下-329 pA的内向电流幅度。在V h =-80 mV的富含Cs + 的移液器中，ICC-MY产生电压依赖性的内向电流（VDIC），而硝苯地平（1μM）阻止了VDIC 。因此，我们成功地从豚鼠胃中分离出了c-Kit阳性单个ICC，并发现ICC-MY有效产生了对二氢吡啶敏感的L型电压依赖性Ca 2 + 电流（VDCC L ）。

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