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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Characteristics of K+ Outward Currents in the Cochlear Outer Hair Cells of Circling Mice within the First Postnatal Week
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Characteristics of K+ Outward Currents in the Cochlear Outer Hair Cells of Circling Mice within the First Postnatal Week

机译：产后第一周内循环小鼠耳蜗外毛细胞K +外向电流的特征

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K+ outward currents in the outer hair cells (OHCs) of circling mice (homozygous ( cir / cir ) mice), an animal model for human deafness (DFNB6 type), were investigated using a whole cell patch clamp technique. Littermate heterozygous (+/ cir ) mice of the same age (postnatal day (P) 0 -P6) were used as controls. Similar slow rising K+ currents were observed in both genotypes, but their biophysical and pharmacological properties were quite different. The values of Vhalf for activation were significantly different in the heterozygous (+/ cir ) and homozygous ( cir / cir ) mice (-8.1±2.2 mV, heterozygous (+/ cir ) mice (n=7) and -17.2±4.2 mV, homozygous ( cir / cir ) mice (n=5)). The inactivation curve was expressed by a single first order Boltzmann equation in the homozygous ( cir / cir ) mice, while it was expressed by a sum of two first order Boltzmann equations in the heterozygous (+/ cir ) mice. The K+ current of homozygous ( cir / cir ) mice was more sensitive to TEA in the 1 to 10 mM range, while the 4-AP sensitivities were not different between the two genotypes. Removal of external Ca2+ did not affect the K+ currents in either genotype, indicating that the higher sensitivity of K+ current to TEA in the homozygous ( cir / cir ) mice was not due to an early expression of Ca2+ activated K+ channels. Our results suggest that the K+ outward current of developing homozygous ( cir / cir ) mice OHCs is different in both biophysical and pharmacological aspects than that of heterozygous (+/ cir ) mice.
机译：使用全细胞贴片研究了盘旋小鼠（纯合（cir / cir）小鼠）（人耳聋的动物模型）（DFNB6型）的外毛细胞（OHC）中的K + 外向电流钳技术。使用相同年龄（产后天数（P）0 -P6）的同伴杂合子（+ / cir）作为对照。在两种基因型中均观察到相似的缓慢上升的K + 电流，但它们的生物物理和药理特性完全不同。在杂合子（+ / cir）和纯合子（cir / cir）（-8.1±2.2 mV，杂合子（+ / cir）的小鼠（n = 7）和-17.2±4.2 mV纯合（cir / cir）小鼠（n = 5））。灭活曲线由纯合子（cir / cir）小鼠中的一个一阶Boltzmann方程表示，而它由杂合性（+ / cir）小鼠中的两个一阶Boltzmann方程之和表示。在1至10 mM范围内，纯合（cir / cir）小鼠的K + 电流对TEA更为敏感，而两种基因型之间的4-AP敏感性没有差异。去除外部Ca 2 + 不会影响任一基因型的K + 电流，表明K + 电流对TEA的敏感性更高。纯合（cir / cir）小鼠不是由于Ca 2 + 激活的K + 通道的早期表达。我们的结果表明，发育中的纯合（cir / cir）小鼠OHCs的K + 外向电流在生物物理和药理学方面均与杂合（+ / cir）小鼠不同。

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