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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >NgR1 Expressed in P19 Embryonal Carcinoma Cells Differentiated by Retinoic Acid Can Activate STAT3
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NgR1 Expressed in P19 Embryonal Carcinoma Cells Differentiated by Retinoic Acid Can Activate STAT3

机译：维甲酸诱导分化的P19胚胎癌细胞中的NgR1可以激活STAT3。

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摘要

NgR1, a Nogo receptor, is involved in inhibition of neurite outgrowth and axonal regeneration and regulation of synaptic plasticity. P19 embryonal carcinoma cells were induced to differentiate into neuron-like cells using all trans-retinoic acid and the presence and/or function of cellular molecules, such as NgR1, NMDA receptors and STAT3, were examined. Neuronally differentiated P19 cells expressed the mRNA and protein of NgR1, which could stimulate the phosphorylation of STAT3 when activated by Nogo-P4 peptide, an active segment of Nogo-66. During the whole period of differentiation, mRNAs of all of the NMDA receptor subtypes tested (NR1, NR2A-2D) were consistently expressed, which meant that neuronally differentiated P19 cells maintained some characteristics of neurons, especially central nervous system neurons. Our results suggests that neuronally differentiated P19 cells expressing NgR1 may be an efficient and convenient in vitro model for studying the molecular mechanism of cellular events that involve NgR1 and its binding partners, and for screening compounds that activate or inhibit NgR1.
机译：NgR1是Nogo受体，参与抑制神经突生长和轴突再生以及调节突触可塑性。使用所有反式维甲酸诱导P19胚胎癌细胞分化为神经元样细胞，并检查细胞分子（例如NgR1，NMDA受体和STAT3）的存在和/或功能。神经元分化的P19细胞表达NgR1的mRNA和蛋白，当被Nogo-66的活性片段Nogo-P4肽激活时，可以刺激STAT3的磷酸化。在整个分化过程中，所有测试的NMDA受体亚型（NR1，NR2A-2D）的mRNA始终表达，这意味着神经元分化的P19细胞保持了神经元的某些特征，尤其是中枢神经系统神经元。我们的结果表明，表达NgR1的神经元分化的P19细胞可能是一种有效且方便的体外模型，用于研究涉及NgR1及其结合伴侣的细胞事件的分子机制，并筛选激活或抑制NgR1的化合物。

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