It is well known that cigarette smoke can cause erectile dysfunction by affecting the penile vascular system. However, the exact effects of nicotine on the corpus cavernosum remains poorly understood. Nicotine has been reported to cause relaxation of the corpus cavernosum; it has also been reported to cause both contraction and relaxation. Therefore, high concentrations of nicotine were studied in strips from the rabbit corpus cavernosum to better understand its effects. The proximal penile corpus cavernosal strips from male rabbits weighing approximately 4 kg were used in organ bath studies. Nicotine in high concentrations (10-5~10-4 M) produced dose-dependent contractions of the corpus cavernosal strips. The incubation with 10-5 M hexamethonium (nicotinic receptor antagonist) significantly inhibited the magnitude of the nicotine associated contractions. The nicotine-induced contractions were not only significantly inhibited by pretreatment with 10-5 M indomethacin (nonspecific cyclooxygenase inhibitor) and with 10-6 M NS-398 (selective cyclooxygenase inhibitor), but also with 10-6 M Y-27632 (Rho kinase inhibitor). Ozagrel (thromboxane A2 synthase inhibitor) and SQ-29548 (highly selective TP receptor antagonist) pretreatments significantly reduced the nicotine-induced contractile amplitude of the strips. High concentrations of nicotine caused contraction of isolated rabbit corpus cavernosal strips. This contraction appeared to be mediated by activation of nicotinic receptors. Rho-kinase and cyclooxygenase pathways, especially cyclooxygenase-2 and thromboxane A2, might play a pivotal role in the mechanism associated with nicotine-induced contraction of the rabbit corpus cavernosum.
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机译：众所周知，香烟烟雾会通过影响阴茎血管系统而导致勃起功能障碍。但是，尼古丁对海绵体的确切作用仍然知之甚少。据报道，尼古丁会引起海绵体的松弛。据报道它还会引起收缩和松弛。因此，对兔海绵体条中的高浓度尼古丁进行了研究，以更好地了解其作用。来自重约4kg的雄性兔子的近端阴茎海绵体海绵体带被用于器官浴研究。高浓度（10 -5 〜10 -4 M）的尼古丁产生海绵体海绵体带的剂量依赖性收缩。与10 -5 M六甲铵盐（烟碱样受体拮抗剂）一起孵育可显着抑制尼古丁相关收缩的幅度。尼古丁诱导的收缩不仅通过用10 -5 M消炎痛（非特异性环氧合酶抑制剂）和10 -6 M NS-398（选择性环氧合酶抑制剂）预处理得到明显抑制。 ），也可以使用10 -6 M Y-27632（Rho激酶抑制剂）。奥扎格雷（血栓烷A2合酶抑制剂）和SQ-29548（高选择性TP受体拮抗剂）预处理显着降低了尼古丁引起的条带收缩幅度。高浓度的尼古丁会引起离体的兔海绵体条收缩。这种收缩似乎是由烟碱样受体的激活介导的。 Rho激酶和环氧合酶途径，尤其是环氧合酶2和血栓烷A 2 ，可能在尼古丁引起的兔海绵体收缩相关机制中起关键作用。
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