Quantification of molecules in 1H-NMR metabolomics with formate as a concentration standard

Itiro Ando; Takuo Hirose; Tadashi Nemoto; Kazuhito Totsune; Yutaka Imai; Kazuhisa Takeuchi; Masako Fujiwara

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Quantification of molecules in 1H-NMR metabolomics with formate as a concentration standard

机译：以甲酸盐为浓度标准品定量 1 H-NMR代谢组学中的分子

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Quantitative analysis of metabolites is important in 1H-nuclear magnetic resonance (NMR)-based metabolomics of plasma. Human plasma contains a high density of proteins which heavily adsorb the commonly-used standard compound of sodium 3-(trimethylsilyl) propionate 2, 2, 3, 3-d₄ (TSP). We have evaluated calcium formate as an alternative standard in 1D single-pulse 1H-NMR spectra to quantify plasma metabolites. Formate did not interact with either plasma metabolites or proteins under adequate conditions. Linear relations between the signal intensities and the added formate have been demonstrated in 1H spectra. The quantifications of glucose and creatinine by this method have shown good accordance with biochemical analysis. Calcium formate is applicable as a concentration standard to NMR metabolomics of plasma.

机译：代谢物的定量分析在基于 1 H-核磁共振（NMR）的血浆代谢组学中很重要。人体血浆中含有高密度的蛋白质，这些蛋白质会大量吸附3-（三甲基甲硅烷基）丙酸钠2、2、3、3-d _{4 （TSP）常用的标准化合物。我们已经评估了甲酸钙作为一维单脉冲 1 H-NMR谱中的替代标准品以定量血浆代谢产物的能力。在适当条件下，甲酸酯既不与血浆代谢物也不与蛋白质相互作用。在 1 H光谱中已经证明了信号强度与所加甲酸盐之间的线性关系。通过该方法对葡萄糖和肌酐的定量显示出与生化分析的良好一致性。甲酸钙可用作血浆NMR代谢组学的浓度标准。}

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《The Journal of toxicological sciences》 |2010年第2期|共4页
作者
Itiro Ando; Takuo Hirose; Tadashi Nemoto; Kazuhito Totsune; Yutaka Imai; Kazuhisa Takeuchi; Masako Fujiwara;
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1. Quantification of molecules in1H-NMR metabolomics with formate as a concentration standard [J] . AndoI., HiroseT., NemotoT., The Journal of toxicological sciences . 2010,第2期

机译：以甲酸盐为浓度标准的1 H-NMR代谢组学中的分子定量
2. Urine Metabolomics by ~1H-NMR Spectroscopy Indicates Associations between Serum 3,5-T_2 Concentrations and Intermediary Metabolism in Euthyroid Humans [J] . Maik Pietzner, Georg Homuth, Kathrin Budde, European thyroid journal . 2015,第S1期

机译：通过〜1H-NMR光谱进行的尿液代谢组学表明，甲状腺正常人血清3,5-T_2浓度与中间代谢之间存在关联
3. 1H-NMR quantification of major saccharides in acai raw materials: a comparison of the internal standard methodology with the absolute intensity qNMR method. [J] . Sterling C., Crouch R., Russell D. J., Phytochemical Analysis . 2013,第6期

机译：acai原料中主要糖类的 1 H-NMR定量：内标方法与绝对强度qNMR方法的比较。
4. QconCAT technology yielding molecules per cell: accurate quantification of absolute enzyme concentrations in yeast via nano-UPLC-MS [C] . Kathleen M Carroll, Deborah M Simpson, Claire E Eyers, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：QConcat技术得到每种细胞分子：通过纳米UPLC-MS准确定量酵母中的绝对酶浓度
5. 1H-NMR Metabolomics Characterizes Transition from Ebb to Flow in a Porcine Model of Smoke Inhalation and Severe Burn Injury [D] . Hendrickson, Cole Grant. 2018

机译：1H-NMR代谢组学表征了烟雾吸入和严重烧伤的猪模型中从退潮到血流的转变
6. Urine Metabolomics by 1H-NMR Spectroscopy Indicates Associations between Serum 35-T2 Concentrations and Intermediary Metabolism in Euthyroid Humans [O] . Maik Pietzner, Georg Homuth, Kathrin Budde, 2015

机译：通过1H-NMR光谱进行的尿液代谢组学表明正常人甲状腺中血清35-T2浓度与中间代谢之间的关联
7. Urine Metabolomics by 1H-NMR Spectroscopy Indicates Associations between Serum 3,5-T2 Concentrations and Intermediary Metabolism in Euthyroid Humans [O] . Maik Pietzner, Georg Homuth, Kathrin Budde, 2015

机译：1H-NMR光谱的尿代谢组合表明血清3,5-T2浓度与Euthyroid人类中中间代谢之间的关联

Quantification of molecules in 1H-NMR metabolomics with formate as a concentration standard

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