...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of toxicological sciences >Synthesized glucocorticoid, dexamethasone regulates the expressions of β2-adrenoceptor and glucocorticoid receptor mRNAs but not proteins in slow-twitch soleus muscle of rats
【24h】

Synthesized glucocorticoid, dexamethasone regulates the expressions of β2-adrenoceptor and glucocorticoid receptor mRNAs but not proteins in slow-twitch soleus muscle of rats

机译：合成的糖皮质激素地塞米松调节慢速比目鱼比目鱼肌中β 2 -肾上腺素受体和糖皮质激素受体mRNA的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Effects of synthesized glucocorticoid, dexamethasone (DEX, dose = 1.0 mg/kg body weight/day for 10 days) on the expressions of β2-adrenoceptor (AR) and glucocorticoid receptor (GR) were studied in fast-twitch (extensor digitorum longus (EDL)) and slow-twitch fiber-rich (soleus(SOL)) muscles of rats. DEX decreased the expression of β2-AR mRNA in SOL muscle without changing that in EDL muscle. The expression of β2-AR protein in EDL and SOL muscles was not affected by DEX. DEX-induced decreased action of the expression of GR mRNA was much greater in SOL muscle than in EDL muscle. However, there were no differences in the expression of GR protein in EDL and SOL muscles. DEX also decreased mRNA expression of cAMP response element binding protein (CREB, transcription factor of β2-AR mRNA) in SOL muscle, whereas increased that in EDL muscle. Further, DEX tended to increase mRNA expressions of post-transcription factors of β2-AR mRNA in EDL muscle without changing those in SOL muscle. These results demonstrated that the expressions of β2-AR and GR are regulated at mRNA levels but not protein levels by DEX. Further, these results also suggest that DEX-induced decrease in the expression of β2-AR mRNA in slow-twitch fiber-rich SOL muscle is associated with the transcriptional regulations.
机译：研究了合成的糖皮质激素地塞米松（DEX，剂量= 1.0 mg / kg体重/天，持续10天）对β 2 肾上腺素受体（AR）和糖皮质激素受体（GR）表达的影响。大鼠快速抽搐（指长伸肌（EDL））和纤维慢抽肌（比目鱼肌（SOL））。 DEX降低了SOL肌肉中β 2 -AR mRNA的表达，而没有改变EDL肌肉中的表达。 β 2 -AR蛋白在EDL和SOL肌肉中的表达不受DEX影响。 DEX诱导的GR mRNA表达的降低作用在SOL肌肉中比在EDL肌肉中大得多。但是，EDL和SOL肌肉中GR蛋白的表达没有差异。 DEX还降低了SOL肌肉中cAMP反应元件结合蛋白（CREB，β 2 -AR mRNA的转录因子）的mRNA表达，而EDL肌肉中的表达增加。此外，DEX倾向于增加EDL肌肉中β 2 -AR mRNA转录后因子的mRNA表达，而不改变SOL肌肉中的表达。这些结果表明，DEX调节β 2 -AR和GR的表达在mRNA水平而非蛋白水平。此外，这些结果还表明，DEX诱导的富含慢肌纤维的SOL肌肉中β 2 -AR mRNA表达的降低与转录调控有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号