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首页> 外文期刊>The Journal of toxicological sciences >Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: a retrospective study on fatal cases in Thailand
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Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: a retrospective study on fatal cases in Thailand

机译：利用核ITS区域和肽毒素对有毒蘑菇进行分子鉴定：泰国致命病例的回顾性研究

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Cases of mushroom poisoning in Thailand have increased annually. During 2008 to 2014, the cases reported to the National Institute of Health included 57 deaths; at least 15 died after ingestion of amanitas, the most common lethal wild mushrooms inhabited. Hence, the aims of this study were to identify mushroom samples from nine clinically reported cases during the 7-year study period based on nuclear ITS sequence data and diagnose lethal peptide toxins using a reversed phase LC-MS method. Nucleotide similarity was identified using BLAST search of the NCBI database and the Barcode of Life Database (BOLD). Clade characterization was performed by maximum likelihood and Bayesian phylogenetic approaches. Based on BLAST and BOLD reference databases our results yielded high nucleotide similarities of poisonous mushroom samples to A. exitialis and A. fuliginea . Detailed phylogenetic analyses showed that all mushroom samples fall into their current classification. Detection of the peptide toxins revealed the presence of amatoxins and phallotoxins in A. exitialis and A. fuliginea . In addition, toxic α-amanitin was identified in a new provisional species, Amanita sp.1, with the highest toxin quantity. Molecular identification confirmed that the mushrooms ingested by the patients were members of the lethal amanitas in the sections Amanita and Phalloideae . In Thailand, the presence of A. exitialis was reported here for the first time and all three poisonous mushroom species provided new and informative data for clinical studies.
机译：泰国的蘑菇中毒案件逐年增加。在2008年至2014年期间，向国立卫生研究院报告的病例包括57例死亡。食用鹅膏菌（居住着最常见的致命野生蘑菇）后，至少有15人死亡。因此，本研究的目的是根据核ITS序列数据从7年研究期间的9例临床报告病例中鉴定蘑菇样品，并使用反相LC-MS方法诊断致命肽毒素。使用NCBI数据库和生命条形码（BOLD）的BLAST搜索来鉴定核苷酸相似性。进化枝的鉴定是通过最大似然法和贝叶斯系统进化方法进行的。基于BLAST和BOLD参考数据库，我们的结果得到了有毒蘑菇样品与硬皮曲霉和黄曲霉的核苷酸高度相似性。详细的系统发育分析表明，所有蘑菇样品均属于当前分类。对肽毒素的检测显示出A. exitialis和A. fuliginea中有amatoxins和phallotoxins。此外，在一种新的临时物种（Amanita sp.1）中发现了毒性最高的α-amanitin。分子鉴定证实患者摄入的蘑菇是鹅膏菌和伞形科的致死鹅膏菌的成员。在泰国，首次报道了存在沙门氏菌的情况，这三种有毒蘑菇物种均为临床研究提供了新的和有益的数据。

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