...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of toxicological sciences >Pharmacological assessment of ARTCEREB? irrigation and perfusion solution for cerebrospinal surgery using primary cultures of rat brain cells
【24h】

Pharmacological assessment of ARTCEREB? irrigation and perfusion solution for cerebrospinal surgery using primary cultures of rat brain cells

机译：利用大鼠脑细胞原代培养ARTCEREB ？冲洗和灌注溶液进行脑脊髓手术的药理学评估

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

ARTCEREB? irrigation and perfusion solution (Artcereb), an ethical pharmaceutical, is typically applied inside the skull and spinal cavity as artificial fluid. Artcereb is composed of glucose and electrolytes (Na+, K+, Mg2+, Ca2+, Cl-, HCO3- and P) and has a pH of 7.3. An in vitro assessment of the effects of Artcereb on cell culture of rat fetal astrocytes or rat fetal brain cells was performed in comparison with normal saline and lactated Ringer’s solutions. Furthermore, the effects of Artcereb on cell culture of rat fetal brain cells were also assessed in comparison with Krebs bicarbonate solution. Cell function after exposure to Artcereb was assessed based on 3H-thymidine incorporation activity. Cell function after exposure to Artcereb and lactated Ringer’s solution in primary cultures of rat fetal astrocytes remained unaffected when compared to that after exposure to normal saline. Cell function after exposure to Artcereb in a primary culture of rat brain cells remained unaffected as compared to that after exposure to normal saline and lactated Ringer’s solution. However, function decreased after exposure to a modified Artcereb formulation lacking bicarbonate, thus confirming that the presence of bicarbonate is essential for the Artcereb formulation.
机译：ARTCEREB ？冲洗和灌注溶液（Artcereb）是一种合乎道德的药物，通常作为人工液体应用在颅骨和脊髓腔内。 Artcereb由葡萄糖和电解质（Na + ，K + ，Mg 2 + ，Ca 2 + ， Cl -，HCO 3 -和P），pH值为7.3。与生理盐水和乳酸林格溶液比较，对Artcereb对大鼠胎儿星形胶质细胞或大鼠胎儿脑细胞的细胞培养的影响进行了体外评估。此外，与克雷布斯碳酸氢盐溶液相比，还评估了Artcereb对大鼠胎儿脑细胞细胞培养的影响。基于 3 H-胸腺嘧啶核苷掺入活性评估暴露于Artcereb后的细胞功能。与暴露于生理盐水后相比，暴露于Artcereb和乳酸林格氏液的大鼠胎儿星形胶质细胞原代培养物中的细胞功能未受影响。与暴露于生理盐水和乳酸林格氏液后相比，暴露于Artcereb的大鼠脑细胞原代培养物中的细胞功能未受影响。但是，暴露于缺乏碳酸氢盐的改良Artcereb制剂后，功能降低，因此证实碳酸氢盐的存在对于Artcereb制剂至关重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号