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首页> 外文期刊>The Journal of Reproduction and Development >Glutathione treatment of Japanese Black bull sperm prior to intracytoplasmic sperm injection promotes embryo development
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Glutathione treatment of Japanese Black bull sperm prior to intracytoplasmic sperm injection promotes embryo development

机译：谷胱甘肽治疗日本黑牛精子在胞浆内注射精子可促进胚胎发育

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Intracytoplasmic sperm injection (ICSI) was expected to enable more efficient use of sperm from sires with preferable genetic traits and result in a generation containing a larger number of offspring with superior genetic characteristics in livestock. However, the efficiency of the early development of embryos produced by ICSI is still far from satisfactory in cattle. The present study aimed to investigate the effects of the treatment of cryopreserved sperm with glutathione (GSH) on the early development of embryos produced by ICSI in Japanese Black cattle. Moreover, the disulfide bond state and mitochondrial function were investigated in the sperm treated with GSH to confirm the effectiveness of the abovementioned treatment. We also investigated the effect of 7% ethanol activation treatment on the developmental ability of ICSI embryos using GSH-treated sperm. There was no effect on the blastocyst rate from the activation treatment. When sperm-injected oocytes were cultured in vitro , the treatment with GSH significantly improved the early development of embryos. Specifically, the rates of embryos reaching the 4–8-cell stage and blastocyst stage were significantly higher in ICSI with GSH-treated sperm (71.4% and 31.0%, respectively) than that with the control sperm (36.6% and 7.0%, respectively). Moreover, the GSH-treated sperm treatment significantly decreased the number of disulfide bonds in the sperm head (as shown by monobromobimane staining) and enhanced the mitochondrial function in the sperm middle piece (as shown by Rhodamine 123 staining and the adenosine triphosphate-dependent bioluminescence assay). Based on these results, we suggest that the treatment of cryopreserved sperm with GSH might contribute to the improvement of ICSI techniques for the production of blastocysts in Japanese Black cattle.
机译：期望通过胞浆内精子注射（ICSI）来更有效地利用具有良好遗传特性的公羊的精子，并产生具有大量具有优良遗传特性的后代的后代。但是，ICSI产生的胚胎早期发育的效率在牛中仍然远远不能令人满意。本研究旨在探讨用谷胱甘肽（GSH）处理冷冻保存的精子对日本黑牛ICSI产生的胚胎早期发育的影响。此外，研究了用GSH处理的精子中的二硫键状态和线粒体功能，以确认上述治疗的有效性。我们还研究了7％的乙醇活化处理对使用GSH处理的精子对ICSI胚胎发育能力的影响。活化处理对胚泡率没有影响。当在体外培养注射精子的卵母细胞时，用GSH处理可显着改善胚胎的早期发育。具体而言，ICSI的GSH处理精子的胚胎达到4-8细胞期和胚泡期的比率显着高于对照精子（分别为36.6％和7.0％）（分别为71.4％和31.0％）。 ）。此外，经GSH处理的精子处理显着减少了精子头部中的二硫键数量（如单溴双马胺染色所示），并增强了精子中段的线粒体功能（如若丹明123染色和依赖三磷酸腺苷的生物发光所示）分析）。根据这些结果，我们建议用GSH处理冷冻保存的精子可能有助于改善ICSI技术，以生产日本黑牛的胚泡。

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